FZD8 Antikörper (C-Term)

Produktnummer ABIN185522

Der Ziege Polyklonal Anti-FZD8-Antikörper wurde für WB, ELISA, IHC, IF und IEM validiert. Er ist geeignet, FZD8 in Proben von Human zu detektieren.

Kurzübersicht für FZD8 Antikörper (C-Term) (ABIN185522)

Target: FZD8 (Frizzled Family Receptor 8 (FZD8))

Reaktivität: Human

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser FZD8 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoelectron Microscopy (IEM)

Güteklasse: Verified

Produktdetails anti-FZD8 Antikörper

Bindungsspezifität: C-Term

Verwendungszweck: FZD8 / frizzled 8

Sequenz: SYPKQMPLSQ V

Kreuzreaktivität: Rind (Kuh), Hund, Human, Maus, Ratte

Aufreinigung: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-SYPKQMPLSQV, from the C Terminus of the protein sequence according to NP_114072.1.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Immunohistochemistry: Paraffin embedded Human Pancreas. Recommended concentration: 3 μg/mL.

Western Blot: Approx 75 kDa band observed in Human Pancreas lysates (calculated MW of 73.3 kDa according to NP_114072.1). Recommended concentration: 1-3 μg/mL. Primary incubation was 1 hour. This antibody has been successfully used in WB on Mouse: Zhao B e

Peptide ELISA: antibody detection limit dilution 1:32000.

Kommentare:

Immunofluorescence: This antibody has been successfully used in IF on Mouse: Zhao B et al. (2019) PMID: 21321602.

Additional validation: This antibody was successfully used in Electron microscopy (immunogold staining):

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Minimize freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Details zu FZD8

Target: FZD8 (Frizzled Family Receptor 8 (FZD8))

Andere Bezeichnung: FZD8

Hintergrund: FZD8, frizzled homolog 8 (Drosophila) , HGNC:4046, FZ-8, hFZ8 , frizzled (Drosophila) homolog 8, frizzled 8

Gen-ID: 8325, 14370

NCBI Accession: NP_114072

Pathways: WNT Signalweg