KPNB1 Antikörper (C-Term)

Produktnummer ABIN185516

Der Ziege Polyklonal Anti-KPNB1-Antikörper wurde für WB, IHC, ELISA, FACS und IF validiert. Er ist geeignet, KPNB1 in Proben von Human und Ratte zu detektieren.

Kurzübersicht für KPNB1 Antikörper (C-Term) (ABIN185516)

Target: KPNB1 (Karyopherin (Importin) beta 1 (KPNB1))

Reaktivität: Human, Ratte

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser KPNB1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Flow Cytometry (FACS), Immunofluorescence (IF)

Güteklasse: Verified

Produktdetails anti-KPNB1 Antikörper

Bindungsspezifität: C-Term

Verwendungszweck: Karyopherin (importin) beta 1

Sequenz: RRSKTNKAKT LAT

Kreuzreaktivität: Rind (Kuh), Hund, Human, Maus, Ratte

Aufreinigung: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-RRSKTNKAKTLAT, from the C Terminus of the protein sequence according to NP_002256.2.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Immunohistochemistry: Paraffin embedded Human Spleen. Recommended concentration: 5 μg/mL.

Western Blot: Approx 90-95- kDa band observed in lysates of cell lines A431, Daudi, HEK293, HeLa, Jurkat, Kelly and KNRK (calculated MW of 97.2 kDa according to Human NP_002256.2 and Rat NP_058759.2). Recommended concentration: 0.01-0.1 μg/mL. Primary incu

Peptide ELISA: antibody detection limit dilution 1:32000.

Kommentare:

Immunofluorescence: Strong expression of the protein seen in U2OS cells. Recommended concentration: 10μg/ml.

Flow Cytometry: Flow cytometric analysis of A431 cells. Recommended concentration: 10ug/ml.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Minimize freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Details zu KPNB1

Target: KPNB1 (Karyopherin (Importin) beta 1 (KPNB1))

Andere Bezeichnung: KPNB1

Hintergrund: KPNB1, karyopherin (importin) beta 1, HGNC:6400, IMB1, IPOB, Impnb, MGC2155, MGC2156, MGC2157, NTF97, importin 90, importin beta-1 subunit, karyopherin beta 1, nuclear factor p97, KapB1, IPO1

Gen-ID: 3837, 16211, 24917

NCBI Accession: NP_002256

Pathways: Protein targeting to Nucleus