CBX3 Antikörper (Internal Region)

Produktnummer ABIN185493

Dieses Anti-CBX3-Antikörper ist ein Ziege Polyklonal-Antikörper zur Detektion von CBX3 in WB, IHC, ELISA, IF und FACS. Geeignet für Human und Maus. Dieses Primary Antibody wurde in 1 Publikation zitiert.

Kurzübersicht für CBX3 Antikörper (Internal Region) (ABIN185493)

Target: CBX3 (Chromobox Homolog 3 (CBX3))

Reaktivität: Human, Maus

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser CBX3 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS)

Güteklasse: Verified

Produktdetails anti-CBX3 Antikörper

Bindungsspezifität: Internal Region

Verwendungszweck: CBX3 / HP1 Gamma

Sequenz: EAFLNSQKAG KEKD

Spezifität: No cross-reactivity expected with HP1-alpha and HP1-beta. Reported variants represent identical protein: NP_009207.2, NP_057671.2

Kreuzreaktivität: Rind (Kuh), Hund, Human, Maus, Ratte

Aufreinigung: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-EAFLNSQKAGKEKD, from the internal region of the protein sequence according to NP_009207.2, NP_057671.2.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Immunohistochemistry: In paraffin embedded Human Placenta shows strong nuclear staining in trophoblasts. Recommended concentration: 2-3 μg/mL.

Western Blot: Approx. 20 kDa band observed in lysates of cell lines A431, HeLa, HepG2, NIH3T3 and NSO (calculated MW of 20.8 kDa according to NP_009207.2 and NP_057671.2). Recommended concentration: 0.3-1.0 μg/mL.

Peptide ELISA: antibody detection limit dilution 1:32000.

Kommentare:

Immunofluorescence: Strong expression of the protein seen in HeLa and U2OS cells. Recommended concentration: 10μg/ml.

Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concentration: 10ug/ml.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Minimize freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Referenzen für anti-CBX3 Antikörper (ABIN185493)

Di Cello, Shin, Harbom, Brayton: "Knockdown of HMGA1 inhibits human breast cancer cell growth and metastasis in immunodeficient mice." in: Biochemical and biophysical research communications, Vol. 434, Issue 1, pp. 70-4, (2013) (PubMed).

Details zu CBX3

Target: CBX3 (Chromobox Homolog 3 (CBX3))

Andere Bezeichnung: CBX3

Hintergrund: CBX3, HP1-GAMMA, chromobox homolog 3 (HP1 gamma homolog, Drosophila) , HGNC:1553, HECH, HP1Hs-gamma , HP1 gamma homolog, chromobox homolog 3, chromobox homolog 3 (Drosophila HP1 gamma), heterochromatin protein HP1 gamma, heterochromatin-like protein 1

Gen-ID: 11335, 12417, 297093

NCBI Accession: NP_009207