NPY5R Antikörper (Internal Region)

Produktnummer ABIN185409

Dieses Anti-NPY5R-Antikörper ist ein Ziege Polyklonal-Antikörper zur Detektion von NPY5R in WB, IHC, ELISA und IP. Geeignet für Human und Hamster. Dieses Primary Antibody wurde in 2+ Publikationen zitiert.

Kurzübersicht für NPY5R Antikörper (Internal Region) (ABIN185409)

Target: NPY5R (Neuropeptide Y Receptor Y5 (NPY5R))

Reaktivität: Human, Hamster

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser NPY5R Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunoprecipitation (IP)

Güteklasse: Verified

Produktdetails anti-NPY5R Antikörper

Bindungsspezifität: Internal Region

Verwendungszweck: Neuropeptide Y receptor Y5

Sequenz: GLSNKENRLE EN

Kreuzreaktivität: Hund, Human, Maus, Ratte

Aufreinigung: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-GLSNKENRLEEN, from the internal region of the protein sequence according to NP_006165.1.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Immunohistochemistry: In paraffin embedded Human Intestine shows staining of ganglion cells. Recommended concentration, 4-6 μg/mL.

Western Blot: Approx 50 kDa band observed in Human Brain, Breast and Duodenum lysates (calculated MW of 50.7 kDa according to NP_006165.1). Recommended concentration: 0.3-1 μg/mL. Primary incubation was 1 hour. This antibody has been successfully used in W

Peptide ELISA: antibody detection limit dilution 1:32000.

Kommentare:

Immunoprecipitation: Customer feedback showed specific results when this product was used in IP. This antibody has been successfully used in IP: PMID: 30503694.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Minimize freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Referenzen für anti-NPY5R Antikörper (ABIN185409)

Czarnecka, Kitlinska: "Cell Surface Protein Detection to Assess Receptor Internalization." in: Bio-protocol, Vol. 6, Issue 20, (2016) (PubMed).

Sheriff, Ali, Yahya, Haider, Balasubramaniam, Amlal: "Neuropeptide Y Y5 receptor promotes cell growth through extracellular signal-regulated kinase signaling and cyclic AMP inhibition in a human breast cancer cell line." in: Molecular cancer research : MCR, Vol. 8, Issue 4, pp. 604-14, (2010) (PubMed).

Details zu NPY5R

Target: NPY5R (Neuropeptide Y Receptor Y5 (NPY5R))

Andere Bezeichnung: NPY5R

Hintergrund: Neuropeptide Y receptor Y5 , HGNC:7958, NPY5R, NPYR5

Molekulargewicht: 50.7kDa

Gen-ID: 4889, 18168, 25340

NCBI Accession: NP_006165

Pathways: Feeding Behaviour