CCKBR Antikörper (Internal Region)

Produktnummer ABIN185407

Dieses Ziege Polyklonal-Antikörper erkennt spezifisch CCKBR in WB, ELISA und IHC. Er zeigt eine Reaktivität gegenüber Human und Maus und wurde in 4+ Publikationen erwähnt.

Kurzübersicht für CCKBR Antikörper (Internal Region) (ABIN185407)

Target: CCKBR (Cholecystokinin B Receptor (CCKBR))

Reaktivität: Human, Maus

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser CCKBR Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Güteklasse: Verified

Produktdetails anti-CCKBR Antikörper

Bindungsspezifität: Internal Region

Verwendungszweck: CCKBR

Sequenz: FDGDSDSDSQ SRVRNQ

Kreuzreaktivität: Rind (Kuh), Human, Maus, Ratte

Aufreinigung: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-FDGDSDSDSQSRVRNQ, from the internal region of the protein sequence according to NP_795344.1.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Immunohistochemistry: Paraffin embedded Human Pancreas. Recommended concentration: 4-6 μg/mL.

Western Blot: Approx 48-50 kDa band observed in Mouse Pancreas lysates (calculated MW of 49.2 kDa according to Mouse NP_031653.1) Recommended concentration: 0.3-1 μg/mL. Primary incubation 1 hour at room temperature.

Peptide ELISA: antibody detection limit dilution 1:32000.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Minimize freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Referenzen für anti-CCKBR Antikörper (ABIN185407)

Quattrone, Dewaele, Wozniak, Bauters, Vanspauwen, Floris, Schöffski, Chibon, Coindre, Sciot, Debiec-Rychter: "Promoting role of cholecystokinin 2 receptor (CCK2R) in gastrointestinal stromal tumour pathogenesis." in: The Journal of pathology, Vol. 228, Issue 4, pp. 565-74, (2014) (PubMed).

El-Kouhen, Morisset: "Control of somatostatin (SS) secretion by CCK-1 and CCK-2 receptors' occupation in RIN-14B cells, a rat pancreatic islet cell line." in: Pancreas, Vol. 39, Issue 2, pp. 127-34, (2010) (PubMed).

Berna, Seiz, Nast, Benten, Bläker, Koch, Lohse, Pace: "CCK1 and CCK2 receptors are expressed on pancreatic stellate cells and induce collagen production." in: The Journal of biological chemistry, Vol. 285, Issue 50, pp. 38905-14, (2010) (PubMed).

El-Kouhen, Morisset: "Cholecystokinin and Somatostatin Negatively Affect Growth of the Somatostatin-RIN-14B Cells." in: International journal of endocrinology, Vol. 2009, pp. 875167, (2009) (PubMed).

Details zu CCKBR

Target: CCKBR (Cholecystokinin B Receptor (CCKBR))

Andere Bezeichnung: CCKBR

Hintergrund: CCKBR, cholecystokinin B receptor , HGNC:1571, CCK-B, GASR, CCK2 receptor, cholecystokinin-B receptor/gastrin receptor, gastrin receptor, gastrin\cholecystokinin brain receptor, CCK2R

Gen-ID: 887, 12426, 25706

NCBI Accession: NP_795344

Pathways: Positive Regulation of Peptide Hormone Secretion, Feeding Behaviour