Arrestin 3 Antikörper (Internal Region)

Produktnummer ABIN185395

Dieses Ziege Polyklonal-Antikörper erkennt spezifisch Arrestin 3 in WB, ELISA, IF und FACS. Er zeigt eine Reaktivität gegenüber Human und wurde in 2+ Publikationen erwähnt.

Kurzübersicht für Arrestin 3 Antikörper (Internal Region) (ABIN185395)

Target: Arrestin 3 (ARRB2) (Arrestin, beta 2 (ARRB2))

Reaktivität: Human

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser Arrestin 3 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS)

Güteklasse: Verified

Produktdetails anti-Arrestin 3 Antikörper

Bindungsspezifität: Internal Region

Verwendungszweck: Arrestin beta 2

Sequenz: HDHIPLPRPQ S

Spezifität: This antibody is expected to recognise reported isoforms 1, 2, 3, 5, 6 and 7. No crossreactivity is expected with Arrestin beta 1.

Kreuzreaktivität: Human, Maus, Ratte

Aufreinigung: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-HDHIPLPRPQS, from the internal region of the protein sequence according to NP_004304.1, NP_945355.1.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Western Blot: Approx 45 kDa band observed in lysates of cell lines A549 and U251 and in preliminary testing of KNRK cell lysate (calculated MW of 46.1 kDa according to NP_004304.1 and 44.4 kDa according to NP_945355.1) Recommended concentration: 0.5-1 μg/mL

Peptide ELISA: antibody detection limit dilution 1:64000.

Kommentare:

Immunofluorescence: Strong expression of the protein seen in HeLa cells. Recommended concentration: 10μg/ml.

Flow Cytometry: Flow cytometric analysis of A549 cells. Recommended concentration: 10ug/ml.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Minimize freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Referenzen für anti-Arrestin 3 Antikörper (ABIN185395)

Zakrzewicz, Krasteva, Wilhelm, Dietrich, Wilker, Padberg, Wygrecka, Grau: "Reduced expression of arrestin beta 2 by graft monocytes during acute rejection of rat kidneys." in: Immunobiology, Vol. 216, Issue 7, pp. 854-61, (2011) (PubMed).

Napolitano, Pasqualetti, Usiello, Santini, Pacini, Sciamanna, Errico, Tassone, Di Dato, Martella, Cuomo, Fisone, Bernardi, Mandolesi, Mercuri, Standaert, Pisani: "Dopamine D2 receptor dysfunction is rescued by adenosine A2A receptor antagonism in a model of DYT1 dystonia." in: Neurobiology of disease, Vol. 38, Issue 3, pp. 434-45, (2010) (PubMed).

Details zu Arrestin 3

Target: Arrestin 3 (ARRB2) (Arrestin, beta 2 (ARRB2))

Andere Bezeichnung: ARRB2

Hintergrund: ARRB2, arrestin, beta 2, HGNC:712, ARB2, ARR2, DKFZp686L0365, arrestin beta 2, beta-arrestin 2, BARR2, arrestin 3

Molekulargewicht: 46.1kDa

Gen-ID: 409, 216869, 25388

NCBI Accession: NP_004304, NP_945355, NP_001244257, NP_001244259, NP_001244260

Pathways: Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, cAMP Metabolic Process, Myometrial Relaxation and Contraction, Regulation of Leukocyte Mediated Immunity, Synaptic Membrane, Regulation of G-Protein Coupled Receptor Protein Signaling, CXCR4-mediated Signaling Events, Phototransduction, Thromboxane A2 Receptor Signaling