POMC Antikörper (C-Term)

Produktnummer ABIN185387

Der Ziege Polyklonal Anti-POMC-Antikörper wurde für ELISA, IHC und FACS validiert. Er ist geeignet, POMC in Proben von Human zu detektieren.

Kurzübersicht für POMC Antikörper (C-Term) (ABIN185387)

Target: POMC (Proopiomelanocortin (POMC))

Reaktivität: Human

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser POMC Antikörper ist unkonjugiert

Applikation: ELISA, Immunohistochemistry (IHC), Flow Cytometry (FACS)

Güteklasse: Verified

Produktdetails anti-POMC Antikörper

Bindungsspezifität: C-Term

Verwendungszweck: Proopiomelanocortin / POMC

Sequenz: NAIIKNAYKK GE

Kreuzreaktivität: Human

Aufreinigung: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-NAIIKNAYKKGE, from the C Terminus of the protein sequence according to NP_000930.1, NP_001030333.1.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Immunohistochemistry: In paraffin embedded Human Anterior Pituitary Gland shows vesilulate staining in glandular cells. Recommended concentration: 2-4 μg/mL.

Western Blot: Preliminary testing showed a band at approx 30 kDa in A431 cell lysate after 1 μg/mL antibody staining (calculated MW of 29.4 kDa according to NP_000930.1). Primary incubation 1 hour at room temperature.

Peptide ELISA: antibody detection limit dilution 1:128000.

Kommentare:

Flow Cytometry: Flow cytometric analysis of A431 cells. Recommended concentration: 10ug/ml.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Minimize freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Details zu POMC

Target: POMC (Proopiomelanocortin (POMC))

Andere Bezeichnung: POMC

Hintergrund: POMC, proopiomelanocortin, Precursor of MSH, POC, ACTH, CLIP, adrenocorticotropin/ beta-lipotropin/ alpha-melanocyte stimulating hormone/ beta-melanocyte stimulating, hormone/ beta-endorphin, LPH, NPP, OTTHUMP00000119991, OTTHUMP00000200964, adrenocortic

Gen-ID: 5443

NCBI Accession: NP_000930, NP_001030333

Pathways: Metabolism of Steroid Hormones and Vitamin D, Peptide Hormone Metabolism, Hormone Activity, Feeding Behaviour