AGRP Antikörper (C-Term)

Produktnummer ABIN185385

Dieses Anti-AGRP-Antikörper ist ein Ziege Polyklonal-Antikörper zur Detektion von AGRP in IHC und ELISA. Geeignet für Human.

Kurzübersicht für AGRP Antikörper (C-Term) (ABIN185385)

Target: AGRP (Agouti Related Protein Homolog (Mouse) (AGRP))

Reaktivität: Human

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser AGRP Antikörper ist unkonjugiert

Applikation: Immunohistochemistry (IHC), ELISA

Güteklasse: Verified

Produktdetails anti-AGRP Antikörper

Bindungsspezifität: C-Term

Verwendungszweck: AGRP / Agouti-related protein

Sequenz: CRKLGTAMNP CSRT

Spezifität: This antibody is expected to recognise both reported isoforms (as represented by NP_001129, NP_015531)

Kreuzreaktivität: Hund, Human, Schwein

Aufreinigung: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence CRKLGTAMNPCSRT, from the C Terminus of the protein sequence according to NP_001129.1, NP_015531.1.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Immunohistochemistry: Frozen section of Human Hypothalamus shows staining of a group of neuronal cell bodies in the human infundibular (arcuate) nucleus and their varicose axons. Recommended concentration: 0.05-0.1 μg/mL.

Western Blot: Preliminary experiments gave bands at approx 45 kDa and 24 kDa in Human Brain lysates after 0.1 μg/mL antibody staining. Thiese bands were successfully blocked by incubation with the immunizing peptide. Primary incubation 1 hour at room temper

Peptide ELISA: antibody detection limit dilution 1:32000.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Minimize freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Details zu AGRP

Target: AGRP (Agouti Related Protein Homolog (Mouse) (AGRP))

Andere Bezeichnung: AGRP

Hintergrund: AGRP, ART, AGRT, ASIP2, agouti related protein homolog (mouse), agouti (mouse) related protein, Agouti-related transcript, mouse, homolog of, MGC118963, agouti related protein homolog

Gen-ID: 181

NCBI Accession: NP_001129, NP_015531

Pathways: Feeding Behaviour, Photoperiodism