PAI1 Antikörper (Internal Region)

Produktnummer ABIN185384

Der Ziege Polyklonal Anti-PAI1-Antikörper wurde für WB, IHC und ELISA validiert. Er ist geeignet, PAI1 in Proben von Human zu detektieren. Es ist 1 Publikation verfügbar.

Kurzübersicht für PAI1 Antikörper (Internal Region) (ABIN185384)

Target: PAI1 (SERPINE1) (Plasminogen Activator Inhibitor 1 (SERPINE1))

Reaktivität: Human

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser PAI1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Güteklasse: Verified

Produktdetails anti-PAI1 Antikörper

Bindungsspezifität: Internal Region

Verwendungszweck: PAI1 / SERPINE1

Sequenz: GFKIDDKGMA PALRH

Kreuzreaktivität: Hund, Human

Aufreinigung: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-GFKIDDKGMAPALRH, from the internal region of the protein sequence according to NP_000593.1, NP_001158885.1.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Immunohistochemistry: In paraffin embedded Human Placenta shows strong staining of cytotrophoblasts. Recommended concentration: 4-6 μg/mL. A customer successfully used this product on Human Breast cancer.

Western Blot: Approx 48 kDa band observed in Human Liver and HeLa lysates, while approx. 45 kDa observed in lyates of cell lines A549 and HepG2 (calculated MW of 45.1 kDa according to NP_000593.1). This molecular weight is routinely observed by other source

Peptide ELISA: antibody detection limit dilution 1:64000.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Minimize freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Referenzen für anti-PAI1 Antikörper (ABIN185384)

Kozaczuk, Selmi, Bednarek: "Bacterial expression, purification and angiogenesis-promoting activity of human thymosin ?4." in: Protein expression and purification, Vol. 90, Issue 2, pp. 142-52, (2013) (PubMed).

Details zu PAI1

Target: PAI1 (SERPINE1) (Plasminogen Activator Inhibitor 1 (SERPINE1))

Andere Bezeichnung: SERPINE1

Hintergrund: SERPINE1, PAI1, PAI, PAI-1, PLANH1, serine (or cysteine) proteinase inhibitor, clade E (nexin, plasminogen activator inhibitor type 1), member 1, plasminogen activator inhibitor, type I, serpin peptidase inhibitor, clade E (nexin, plasminogen activator in

Molekulargewicht: 45.1kDa

Gen-ID: 5054

NCBI Accession: NP_000593

Pathways: p53 Signalweg, Cellular Response to Molecule of Bacterial Origin, Carbohydrate Homeostasis, Autophagie, Smooth Muscle Cell Migration