PDE5A Antikörper (C-Term)

Produktnummer ABIN185350

Dieses Ziege Polyklonal-Antikörper erkennt spezifisch PDE5A in WB, ELISA, IF und FACS. Er zeigt eine Reaktivität gegenüber Human.

Kurzübersicht für PDE5A Antikörper (C-Term) (ABIN185350)

Target: PDE5A (phosphodiesterase 5A, cGMP-Specific (PDE5A))

Reaktivität: Human

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser PDE5A Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS)

Güteklasse: Verified

Produktdetails anti-PDE5A Antikörper

Bindungsspezifität: C-Term

Verwendungszweck: PDE5A

Sequenz: LINGESGQAK RN

Spezifität: This antibody is expected to recognise reported isoforms NP_001074.2, NP_236914.2 and NP_246273.2.

Kreuzreaktivität: Rind (Kuh), Hund, Human, Ratte

Aufreinigung: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-LINGESGQAKRN, from the C Terminus of the protein sequence according to NP_001074.2, NP_246273.2, NP_236914.2, (NP_237223).

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Western Blot: In transfected HEK293 transiently expressing PDE5A isoform 2 a band of approx. 95 kDa is observed. This band is not observed in the non-transfected HEK293 (calculated MW of 94.8 kDa according to isoform 2, NP_236914.2). Recommended concentrat

Peptide ELISA: antibody detection limit dilution 1:16000.

Kommentare:

Immunofluorescence: Strong expression of the protein seen in the cytoplasm of HeLa cells. Recommended concentration: 10μg/ml.

Flow Cytometry: Flow cytometric analysis of HEK293 cells. Recommended concentration: 10ug/ml

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Minimize freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Details zu PDE5A

Target: PDE5A (phosphodiesterase 5A, cGMP-Specific (PDE5A))

Andere Bezeichnung: PDE5A

Hintergrund: PDE5A, CN5A, PDE5, PDE5A1, CGB-PDE, phosphodiesterase 5A, cGMP-specific, cGMP-binding cGMP-specific 3',5'-cyclic nucleotide phosphodiesterase

Gen-ID: 8654

NCBI Accession: NP_001074, NP_246273, NP_236914

Pathways: Regulation of G-Protein Coupled Receptor Protein Signaling