GAPDH Antikörper (C-Term)

Produktnummer ABIN185240

Der Ziege Polyklonal Anti-GAPDH-Antikörper wurde für WB, ELISA, IHC und IF validiert. Er ist geeignet, GAPDH in Proben von Human und Ratte zu detektieren. Es sind 10+ Publikationen verfügbar.

Kurzübersicht für GAPDH Antikörper (C-Term) (ABIN185240)

Target: GAPDH (Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH))

Reaktivität: Human, Ratte

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser GAPDH Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF)

Güteklasse: Verified

Produktdetails anti-GAPDH Antikörper

Bindungsspezifität: C-Term

Verwendungszweck: GAPDH (C Terminus) Loading Control

Sequenz: HQVVSSDFNS DT

Spezifität: GAPDH is constitutively expressed in almost all tissues at high levels. It is therefore a useful marker when a loading/positive control is required in western blotting.

Kreuzreaktivität: Hund, Human, Maus, Schwein

Aufreinigung: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-HQVVSSDFNSDT, from the C Terminus of the protein sequence according to NP_002037.2.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Immunohistochemistry: Paraffin embedded Human Pancreas. Recommended concentration: 2.5 μg/mL.

Western Blot: Approx 36 kDa band observed in lysates of cell line HEK293, approx. 37 kDa in Rat Brain lysates and approx.35 kDa in Human Liver and Tonsil lysates, lysates of cell line HeLa and in preliminary testing of Mouse Spleen lysates (calculated MW of

Peptide ELISA: antibody detection limit dilution 1:128000.

Kommentare:

Immunofluorescence: Strong expression of the protein seen in the cytoplasm and vesicles of A549 and HeLa cells. Recommended concentration: 10μg/ml.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Minimize freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Referenzen für anti-GAPDH Antikörper (ABIN185240)

Osei Kuffour, König, Häussinger, Schulz, Münk: "ISG15 Deficiency Enhances HIV-1 Infection by Accumulating Misfolded p53." in: mBio, Vol. 10, Issue 4, (2020) (PubMed).

George, Jacobs: "Minimal effects of spargel (PGC-1) overexpression in a Drosophila mitochondrial disease model." in: Biology open, Vol. 8, Issue 7, (2019) (PubMed).

Hain, Krämer, Linka, Nakhaei-Rad, Ahmadian, Häussinger, Borkhardt, Münk: "IL-2 Inducible Kinase ITK is Critical for HIV-1 Infection of Jurkat T-cells." in: Scientific reports, Vol. 8, Issue 1, pp. 3217, (2019) (PubMed).

Happold, Roth, Silginer, Florea, Lamszus, Frei, Deenen, Reifenberger, Weller: "Interferon-? induces loss of spherogenicity and overcomes therapy resistance of glioblastoma stem cells." in: Molecular cancer therapeutics, Vol. 13, Issue 4, pp. 948-61, (2014) (PubMed).

Happold, Roth, Wick, Schmidt, Florea, Silginer, Reifenberger, Weller: "Distinct molecular mechanisms of acquired resistance to temozolomide in glioblastoma cells." in: Journal of neurochemistry, Vol. 122, Issue 2, pp. 444-55, (2013) (PubMed).

Gramatzki, Herrmann, Happold, Becker, Gulbins, Weller, Tabatabai: "Glioma cell death induced by irradiation or alkylating agent chemotherapy is independent of the intrinsic ceramide pathway." in: PLoS ONE, Vol. 8, Issue 5, pp. e63527, (2013) (PubMed).

Kiepe, Van Der Pas, Ciarmatori, Ständker, Schütt, Hoeflich, Hügel, Oh, Tönshoff: "Defined carboxy-terminal fragments of insulin-like growth factor (IGF) binding protein-2 exert similar mitogenic activity on cultured rat growth plate chondrocytes as IGF-I." in: Endocrinology, Vol. 149, Issue 10, pp. 4901-11, (2008) (PubMed).

Henson, Roberts, Salimi, Vadlamudi, Hamer, Gilmore, Jarskog, Philpot: "Developmental regulation of the NMDA receptor subunits, NR3A and NR1, in human prefrontal cortex." in: Cerebral cortex (New York, N.Y. : 1991), Vol. 18, Issue 11, pp. 2560-73, (2008) (PubMed).

Gerstner, Bremer, Vander Heyden, Lavaute, Yin, Landry: "Brain fatty acid binding protein (Fabp7) is diurnally regulated in astrocytes and hippocampal granule cell precursors in adult rodent brain." in: PLoS ONE, Vol. 3, Issue 2, pp. e1631, (2008) (PubMed).

Nguyen, Pace, Koller: "Mice lacking NKCC1 are protected from development of bacteremia and hypothermic sepsis secondary to bacterial pneumonia." in: The Journal of experimental medicine, Vol. 204, Issue 6, pp. 1383-93, (2007) (PubMed).

Details zu GAPDH

Target: GAPDH (Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH))

Andere Bezeichnung: GAPDH

Hintergrund: GAPDH, glyceraldehyde-3-phosphate dehydrogenase, HGNC:4141, G3PD, GAPD, MGC88685, OTTHUMP00000174431, OTTHUMP00000174432, aging-associated gene 9 protein, glyceraldehyde 3-phosphate dehydrogenase

Gen-ID: 2597

NCBI Accession: NP_002037