ACTR1A Antikörper (C-Term)

Produktnummer ABIN185219

Der Ziege Polyklonal Anti-ACTR1A-Antikörper wurde für WB, ELISA und FACS validiert. Er ist geeignet, ACTR1A in Proben von Human, Maus, Ratte und Hund zu detektieren.

Kurzübersicht für ACTR1A Antikörper (C-Term) (ABIN185219)

Target: ACTR1A (Alpha Centractin (ACTR1A))

Reaktivität: Human, Maus, Ratte, Hund

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser ACTR1A Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Flow Cytometry (FACS)

Güteklasse: Verified

Produktdetails anti-ACTR1A Antikörper

Bindungsspezifität: C-Term

Verwendungszweck: ARP1 homolog A

Sequenz: YEEDGARSIH RKT

Spezifität: This epitope was selected to minimise the chance of cross-reactivity with ACTR1B (YEEDGSRAIHRKT).

Kreuzreaktivität: Rind (Kuh), Hund, Human, Maus, Schwein, Ratte

Aufreinigung: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-YEEDGARSIHRKT, from the C Terminus of the protein sequence according to NP_005727.1.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Western Blot: Approx. 42-45 kDa band observed in lysates of cell lines A431 HEK293, HeLa, MCF7, NIH3T3, KNRK and MDCK, and in preliminary testing of Human and Mouse Adrenal gland and Mouse Stomach lysate (calculated MW of 42.6 kDa according to Human NP_005

Peptide ELISA: antibody detection limit dilution 1:64000.

Kommentare:

Immunofluorescence: A customer reported cytoplasmic staining on A431 cells at a primary ab concentration of 10ug/ml.

Flow Cytometry: Flow cytometric analysis of A431 cells. Recommended concentration: 10ug/ml.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Minimize freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Details zu ACTR1A

Target: ACTR1A (Alpha Centractin (ACTR1A))

Andere Bezeichnung: ACTR1A

Hintergrund: ACTR1A, ARP1, ARP1 actin-related protein 1 homolog A, centractin alpha (yeast), actin-RPV, centractin alpha, ARP1, yeast homolog A, centrosome-associated actin homolog, ARP1 (actin-related protein 1, yeast) homolog A (centractin alpha), CTRN1, FLJ52695, F

Gen-ID: 10121, 54130

NCBI Accession: NP_005727

Pathways: M Phase