IFT74 Antikörper (C-Term)

Produktnummer ABIN185213

Der Ziege Polyklonal Anti-IFT74-Antikörper wurde für ELISA und WB validiert. Er ist geeignet, IFT74 in Proben von Human zu detektieren. Es sind 2+ Publikationen verfügbar.

Kurzübersicht für IFT74 Antikörper (C-Term) (ABIN185213)

Target: IFT74 (Intraflagellar Transport Protein 74 Homolog (IFT74))

Reaktivität: Human

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser IFT74 Antikörper ist unkonjugiert

Applikation: ELISA, Western Blotting (WB)

Güteklasse: Verified

Produktdetails anti-IFT74 Antikörper

Bindungsspezifität: C-Term

Verwendungszweck: CMG1 / CCDC2 / IFT74

Sequenz: KTIVDALHST SGN

Kreuzreaktivität: Human, Ratte

Aufreinigung: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-KTIVDALHSTSGN, from the C Terminus of the protein sequence according to NP_001092692.1, NP_001092693.1, NP_079379.2, AAK77221.1.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Western Blot: Approx 70 kDa band observed in lysates of cell line HEK293 (calculated MW of 69.2 kDa according to AAK77221.1). The observed molecular weight corresponds to earlier findings in literature (Momeni et al, BMC Neurol. 2006 Dec 13,6:44., PMID: 17

Peptide ELISA: antibody detection limit dilution 1:32000.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Minimize freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Referenzen für anti-IFT74 Antikörper (ABIN185213)

Boldt, Mans, Won, van Reeuwijk, Vogt, Kinkl, Letteboer, Hicks, Hurd, Naggert, Texier, den Hollander, Koenekoop, Bennett, Cremers, Gloeckner, Nishina, Roepman, Ueffing: "Disruption of intraflagellar protein transport in photoreceptor cilia causes Leber congenital amaurosis in humans and mice." in: The Journal of clinical investigation, Vol. 121, Issue 6, pp. 2169-80, (2011) (PubMed).

Momeni, Schymick, Jain, Cookson, Cairns, Greggio, Greenway, Berger, Pickering-Brown, Chiò, Fung, Holtzman, Huey, Wassermann, Adamson, Hutton, Rogaeva, St George-Hyslop, Rothstein, Hardiman, Grafman et al.: "Analysis of IFT74 as a candidate gene for chromosome 9p-linked ALS-FTD. ..." in: BMC neurology, Vol. 6, pp. 44, (2007) (PubMed).

Details zu IFT74

Target: IFT74 (Intraflagellar Transport Protein 74 Homolog (IFT74))

Andere Bezeichnung: IFT74

Hintergrund: IFT74, CCDC2, CMG-1, FLJ22621, MGC111562, intraflagellar transport 74 homolog (Chlamydomonas), capillary morphogenesis protein 1, coiled-coil domain containing 2, CMG1

Gen-ID: 80173, 313365

NCBI Accession: NP_001092692, NP_001092693, NP_079379

Pathways: Hedgehog Signalweg, Chromatin Binding