SMUG1 Antikörper (N-Term)

Produktnummer ABIN185204

Der Ziege Polyklonal Anti-SMUG1-Antikörper wurde für ELISA, IF und FACS validiert. Er ist geeignet, SMUG1 in Proben von Human zu detektieren. Es ist 1 Publikation verfügbar.

Kurzübersicht für SMUG1 Antikörper (N-Term) (ABIN185204)

Target: SMUG1 (Single-Strand-Selective Monofunctional Uracil-DNA Glycosylase 1 (SMUG1))

Reaktivität: Human

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser SMUG1 Antikörper ist unkonjugiert

Applikation: ELISA, Immunofluorescence (IF), Flow Cytometry (FACS)

Güteklasse: Verified

Produktdetails anti-SMUG1 Antikörper

Bindungsspezifität: N-Term

Verwendungszweck: SMUG1

Sequenz: PQAFLLGSIH EPA

Kreuzreaktivität: Human

Aufreinigung: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence PQAFLLGSIHEPA-C, from the N Terminus of the protein sequence according to NP_055126.1.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Western Blot: Preliminary testing showed a band at approx 19 kDa in lysate of cell line HeLa after 0.1 μg/mL antibody staining (calculated MW of 19.6 kDa according to NP_001230718.1),. Primary incubation 1 hour at room temperature.

Peptide ELISA: antibody detection limit dilution 1:128000.

Kommentare:

Immunofluorescence: Strong expression of the protein seen in the nuclei of MCF7 and U205 cells. Recommended concentration: 10μg/ml.

Flow Cytometry: Flow cytometric analysis of MCF7 cells. Recommended concentration: 10u

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Minimize freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Referenzen für anti-SMUG1 Antikörper (ABIN185204)

Ma, Au, Waye: "Over-expression of SUMO-1 induces the up-regulation of heterogeneous nuclear ribonucleoprotein A2/B1 isoform B1 (hnRNP A2/B1 isoform B1) and uracil DNA glycosylase (UDG) in hepG2 cells." in: Cell biochemistry and function, Vol. 27, Issue 4, pp. 228-37, (2009) (PubMed).

Details zu SMUG1

Target: SMUG1 (Single-Strand-Selective Monofunctional Uracil-DNA Glycosylase 1 (SMUG1))

Andere Bezeichnung: SMUG1

Hintergrund: SMUG1, single-strand selective monofunctional uracil DNA glycosylase, FDG, HMUDG, UNG3, single-strand-selective monofunctional uracil-DNA glycosylase 1, MGC104370

Gen-ID: 23583

NCBI Accession: NP_055126

Pathways: DNA Reparatur