POLL Antikörper (C-Term)

Produktnummer ABIN185143

Dieses Ziege Polyklonal-Antikörper erkennt spezifisch POLL in WB und ELISA. Er zeigt eine Reaktivität gegenüber Human.

Kurzübersicht für POLL Antikörper (C-Term) (ABIN185143)

Target: POLL (Polymerase (DNA Directed), lambda (POLL))

Reaktivität: Human

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser POLL Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA

Güteklasse: Verified

Produktdetails anti-POLL Antikörper

Bindungsspezifität: C-Term

Verwendungszweck: POLL / POLkappa

Sequenz: LGLPYREPAE RDW

Spezifität: It has been brought to our attention that this product may crossreact with Pol beta (NP_002681), an evolutionarilly related polymerase with a predicted size of 39 kDa. The overall % of identity is not very high (about 32 % in the core) but considering only

Kreuzreaktivität: Human

Aufreinigung: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-LGLPYREPAERDW, from the C Terminus of the protein sequence according to NP_037406.1.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Western Blot: Approx 60 kDa band observed in cell lysates of A549 and approx 60-70 kDa in cell lysates of HepG2 (calculated MW of 63.5 kDa according to Human NP_037406.1). Recommended concentration: 0.5-1 μg/mL.

Peptide ELISA: antibody detection limit dilution 1:32000.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Minimize freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Details zu POLL

Target: POLL (Polymerase (DNA Directed), lambda (POLL))

Andere Bezeichnung: POLL

Hintergrund: POLL, BETA-N, POL-KAPPA, polymerase (DNA directed), lambda, polymerase (DNA-directed), lambda, DNA polymerase kappa (DNA polymerase beta-N), RP11-529I10.1, BETAN, FLJ46002, POLKAPPA, DNA polymerase beta-2, DNA polymerase kappa (DNA polymerase beta-N), DNA

Gen-ID: 27343, 56626

NCBI Accession: NP_037406

Pathways: DNA Reparatur, Production of Molecular Mediator of Immune Response