FARP1 Antikörper (N-Term)

Produktnummer ABIN185104

Produktdetails anti-FARP1 Antikörper

Target: FARP1 (FERM, RhoGEF (ARHGEF) and Pleckstrin Domain Protein 1 (Chondrocyte-Derived) (FARP1))

Bindungsspezifität: N-Term

Reaktivität: Human

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser FARP1 Antikörper ist unkonjugiert

Applikation: ELISA, Immunofluorescence (IF), Flow Cytometry (FACS)

Verwendungszweck: CDEP / FARP1

Sequenz: GEIEQRPTPG SRL

Kreuzreaktivität: Rind (Kuh), Human, Maus, Ratte

Aufreinigung: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Güteklasse: Verified

Immunogen: Peptide with sequence GEIEQRPTPGSRL-C, from the N Terminus of the protein sequence according to NP_005757.1, NP_001001715.2.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Western Blot: Preliminary experiments showed an approx 110-120 kDa band in Human Frontal Cortex lysates (calculated MW of 119 kDa according to NP_ 005757.1).. An additional band was also consistently observed at 40 kDa and was successfully blocked by incuba

Peptide ELISA: antibody detection limit dilution 1:32000.

Kommentare:

Immunofluorescence: Strong expression of the protein seen in the cytoplasm of U2OS and HeLa cells. Recommended concentration: 10μg/ml.

Flow Cytometry: Flow cytometric analysis of HepG2 cells. Recommended concentration:

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Minimize freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Details zu FARP1

Target: FARP1 (FERM, RhoGEF (ARHGEF) and Pleckstrin Domain Protein 1 (Chondrocyte-Derived) (FARP1))

Andere Bezeichnung: FARP1 (FARP1 Produkte)

Synonyme: FARP1 antikoerper, CDEP antikoerper, PLEKHC2 antikoerper, PPP1R75 antikoerper, AW228844 antikoerper, BC030329 antikoerper, Cdep antikoerper, FERM, ARH/RhoGEF and pleckstrin domain protein 1 antikoerper, FERM, RhoGEF (Arhgef) and pleckstrin domain protein 1 (chondrocyte-derived) antikoerper, FARP1 antikoerper, Farp1 antikoerper

Hintergrund: FARP1, FERM, RhoGEF (ARHGEF) and pleckstrin domain protein 1 (chondrocyte-derived), CDEP, chondrocyte-derived ezrin-like protein, RP11-111L24.1, MGC87400, PLEKH2, FERM, RhoGEF, and pleckstrin domain protein 1

Gen-ID: 10160

NCBI Accession: NP_005757, NP_001001715, NP_001273768