Forkhead Box K2 Antikörper (C-Term)

Produktnummer ABIN185049

Dieses Anti-Forkhead Box K2-Antikörper ist ein Ziege Polyklonal-Antikörper zur Detektion von Forkhead Box K2 in WB, ELISA, IF und FACS. Geeignet für Human.

Kurzübersicht für Forkhead Box K2 Antikörper (C-Term) (ABIN185049)

Target: Forkhead Box K2 (FOXK2)

Reaktivität: Human

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser Forkhead Box K2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS)

Güteklasse: Verified

Produktdetails anti-Forkhead Box K2 Antikörper

Bindungsspezifität: C-Term

Verwendungszweck: FOXK2 / ILF (isoform 1)

Sequenz: TPPAAVREKG VQN

Kreuzreaktivität: Human

Aufreinigung: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-TPPAAVREKGVQN, from the C Terminus of the protein sequence according to NP_004505.2.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Western Blot: Approx 80 kDa band observed in nuclear lysates of cell lines HEK293, HeLa and Jurkat (calculated MW of 69.1 kDa according to NP_004505.2.) This molecular weight is routinely observed by other sources and was successfully blocked by incubatio

Peptide ELISA: antibody detection limit dilution 1:32000.

Kommentare:

Immunofluorescence: Strong expression of the protein seen in the nuclei and cytoplasmic/vesicles of U2OS and HeLa cells. Recommended concentration: 10μg/ml.

Flow Cytometry: Flow cytometric analysis of HeLa cells. Recom

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Minimize freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Details zu Forkhead Box K2

Target: Forkhead Box K2 (FOXK2)

Andere Bezeichnung: FOXK2

Hintergrund: ILF1, FOXK2, ILF, ILF-1, interleukin enhancer binding factor 1, cellular transcription factor ILF-1, forkhead box K2

Gen-ID: 3607

NCBI Accession: NP_004505