Forkhead Box K2 Antikörper (C-Term)

Produktnummer ABIN185049

Dieser Anti-Forkhead Box K2 Antikörper ist ein Ziege Polyklonal Antikörper zur Detektion von Forkhead Box K2 in WB, ELISA, IF und FACS. Geeignet für Human.

Kurzübersicht für Forkhead Box K2 Antikörper (C-Term) (ABIN185049)

Target: Forkhead Box K2 (FOXK2)

Reaktivität: Human

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser Forkhead Box K2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS)

Güteklasse: Verified

Produktdetails anti-Forkhead Box K2 Antikörper

Bindungsspezifität: C-Term

Verwendungszweck: FOXK2 / ILF (isoform 1)

Sequenz: TPPAAVREKG VQN

Kreuzreaktivität: Human

Aufreinigung: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-TPPAAVREKGVQN, from the C Terminus of the protein sequence according to NP_004505.2.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Western Blot: Approx 80 kDa band observed in nuclear lysates of cell lines HEK293, HeLa and Jurkat (calculated MW of 69.1 kDa according to NP_004505.2.) This molecular weight is routinely observed by other sources and was successfully blocked by incubatio

Peptide ELISA: antibody detection limit dilution 1:32000.

Kommentare:

Immunofluorescence: Strong expression of the protein seen in the nuclei and cytoplasmic/vesicles of U2OS and HeLa cells. Recommended concentration: 10μg/ml.

Flow Cytometry: Flow cytometric analysis of HeLa cells. Recom

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Minimize freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Details zu Forkhead Box K2

Target: Forkhead Box K2 (FOXK2)

Andere Bezeichnung: FOXK2

Hintergrund: ILF1, FOXK2, ILF, ILF-1, interleukin enhancer binding factor 1, cellular transcription factor ILF-1, forkhead box K2

Gen-ID: 3607

NCBI Accession: NP_004505