REV1 Antikörper (C-Term)

Produktnummer ABIN185012

Der Ziege Polyklonal Anti-REV1-Antikörper wurde für IHC und ELISA validiert. Er ist geeignet, REV1 in Proben von Human zu detektieren.

Kurzübersicht für REV1 Antikörper (C-Term) (ABIN185012)

Target: REV1 (REV1, Polymerase (DNA Directed) (REV1))

Reaktivität: Human

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser REV1 Antikörper ist unkonjugiert

Applikation: Immunohistochemistry (IHC), ELISA

Güteklasse: Verified

Produktdetails anti-REV1 Antikörper

Bindungsspezifität: C-Term

Verwendungszweck: REV1L

Sequenz: VLQQTYGSTL KVT

Spezifität: This antibody is expected to recognise both reported isoforms.

Kreuzreaktivität: Rind (Kuh), Hund, Human, Maus, Ratte

Aufreinigung: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-VLQQTYGSTLKVT, from the C Terminus of the protein sequence according to NP_057400.1, NP_001032961.1.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Immunohistochemistry: In paraffin embedded Human Testis shows nuclear staining in spermatogonia and spermatocytes. Recommended concentration, 4-6 μg/mL.

Western Blot: No signal obtained yet but low background observed in MOLT-4 lysates at up to 1.5 μg/mL. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates?

Peptide ELISA: antibody detection limit dilution 1:8000.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Minimize freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Details zu REV1

Target: REV1 (REV1, Polymerase (DNA Directed) (REV1))

Andere Bezeichnung: REV1

Hintergrund: REV1, REV1L, REV1-like (yeast), REV1, REV1 protein, REV1 (yeast homolog)- like, REV1 homolog (S. cerevisiae), FLJ21523, MGC163283, MGC26225, REV1- like

Gen-ID: 51455, 56210

NCBI Accession: NP_057400, NP_001032961

Pathways: DNA Reparatur, Regulation of Actin Filament Polymerization, Production of Molecular Mediator of Immune Response