PARK7/DJ1 Antikörper (C-Term)

Produktnummer ABIN184951

Dieses Ziege Polyklonal-Antikörper erkennt spezifisch PARK7/DJ1 in WB, IHC, ELISA und IF. Er zeigt eine Reaktivität gegenüber Human, Maus und Ratte.

Kurzübersicht für PARK7/DJ1 Antikörper (C-Term) (ABIN184951)

Target: PARK7/DJ1 (PARK7) (Parkinson Protein 7 (PARK7))

Reaktivität: Human, Maus, Ratte

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser PARK7/DJ1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF)

Güteklasse: Verified

Produktdetails anti-PARK7/DJ1 Antikörper

Bindungsspezifität: C-Term

Verwendungszweck: PARK7 / DJ-1

Sequenz: AAQVKAPLVL KD

Spezifität: Reported variants (NP_001116849.1, NP_009193.2) represent the same protein.

Kreuzreaktivität: Human, Maus, Ratte

Aufreinigung: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-AAQVKAPLVLKD, from the C Terminus of the protein sequence according to NP_001116849.1, NP_009193.2.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Immunohistochemistry: Paraffin embedded Human Testis. Recommended concentration: 7 μg/mL.

Western Blot: Approx 24 kDa band observed in lysates of cell lines HeLa and Jurkat and of Human Brain (Cerebellum, Hippocampus, Frontal Cortex) and rodent Brain lysates (calculated MW of 19.9 kDa according to NP_001116849.1 and of NP_009193.2). Recommended

Peptide ELISA: antibody detection limit dilution 1:128000.

Kommentare:

Immunofluorescence: Strong expression of the protein seen around the nucleus. Dividing cells appear to show enhanced expression. Recommended concentration: 5μg/ml.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Minimize freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Details zu PARK7/DJ1

Target: PARK7/DJ1 (PARK7) (Parkinson Protein 7 (PARK7))

Andere Bezeichnung: PARK7

Hintergrund: PARK7, DJ1, DJ-1, Parkinson disease (autosomal recessive, early onset) 7, oncogene DJ1, CTA-215D11.1, FLJ27376, FLJ34360, FLJ92274, OTTHUMP00000001350, OTTHUMP00000001351, Parkinson disease protein 7, protein DJ-1

Gen-ID: 11315, 57320, 117287

NCBI Accession: NP_009193

Pathways: Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Proton Transport