DPF1 Antikörper (C-Term)

Produktnummer ABIN184922

Der Ziege Polyklonal Anti-DPF1-Antikörper wurde für ELISA und IF validiert. Er ist geeignet, DPF1 in Proben von Human zu detektieren.

Kurzübersicht für DPF1 Antikörper (C-Term) (ABIN184922)

Target: DPF1 (D4, Zinc and Double PHD Fingers Family 1 (DPF1))

Reaktivität: Human

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser DPF1 Antikörper ist unkonjugiert

Applikation: ELISA, Immunofluorescence (IF)

Güteklasse: Verified

Produktdetails anti-DPF1 Antikörper

Bindungsspezifität: C-Term

Verwendungszweck: Neuro-d4 / DPF1

Sequenz: HLKEKASAYI TLT

Spezifität: This antibody is expected to recognize all three reported isoforms (NP_001128627.1, NP_004638.2, NP_001128628.1).

Kreuzreaktivität: Rind (Kuh), Human, Maus, Ratte

Aufreinigung: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-HLKEKASAYITLT, from the C Terminus of the protein sequence according to NP_001128627.1, NP_004638.2, NP_001128628.1.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Western Blot: Preliminary experiments showed a band at approx 38-40 kDa in Human Cerebellum and Frontal Cortex lysates after 0.3-1 μg/mL antibody staining (calculated MW of 37.9 kDa according to NP_001128628.1. Primary incubation 1 hour at room temperature.

Peptide ELISA: antibody detection limit dilution 1:2000.

Kommentare:

Immunofluorescence: Strong expression of the protein seen in the nuclei and weaker staining in the cytoplasm of MCF7 and U2OS cells. Recommended concentration: 10μg/ml.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Minimize freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Details zu DPF1

Target: DPF1 (D4, Zinc and Double PHD Fingers Family 1 (DPF1))

Andere Bezeichnung: DPF1

Hintergrund: DPF1, NEUD4, neuro-d4, D4, zinc and double PHD fingers family 1, Neuro-d4 (rat) homolog, D4, zinc and double PHD fingers, family 1, MGC150428, MGC150429, BAF45b, OTTHUMP00000211615, neuro-d4 homolog

Gen-ID: 8193, 29861, 50545

NCBI Accession: NP_001128627, NP_004638, NP_001128628