Melanophilin Antikörper (C-Term)

Produktnummer ABIN184753

Dieses Ziege Polyklonal-Antikörper erkennt spezifisch Melanophilin in WB, ELISA, IF, ICC und Pull-Down. Er zeigt eine Reaktivität gegenüber Maus und wurde in 4+ Publikationen erwähnt.

Kurzübersicht für Melanophilin Antikörper (C-Term) (ABIN184753)

Target: Melanophilin (MLPH)

Reaktivität: Maus

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser Melanophilin Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC), Pull-Down Assay (Pull-Down)

Güteklasse: Verified

Produktdetails anti-Melanophilin Antikörper

Bindungsspezifität: C-Term

Verwendungszweck: Melanophilin (mouse)

Sequenz: ARHIFAKPVM AQQP

Kreuzreaktivität: Maus, Ratte

Aufreinigung: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-ARHIFAKPVMAQQP, from the C Terminus of the protein sequence according to NP_443748.2.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Western Blot: Approx 80 kDa band observed in Mouse Melanocyte extracts. Recommended for use at 0.5-2 μg/mL. Please note that this product may require overnight incubation of primary and high protein loads. This antibody has been successfully used in WB on

Peptide ELISA: antibody detection limit dilution 1:32000.

Kommentare:

Immunofluorescence: Successfully used at 0.5ug/ml to stain mouse melanocytes. This antibody has been successfully used in IF on Mouse: Figueiredo AC et al., J Biol Chem. 2008 Aug 22,283(34):23209-16. PMID:18559336.
GST-pull do

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Minimize freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Referenzen für anti-Melanophilin Antikörper (ABIN184753)

Sanzà, Evans, Briggs, Cantero, Montoliu, Patel, Sviderskaya, Itzen, Figueiredo, Seabra, Hume: "Nucleotide exchange factor Rab3GEP requires DENN and non-DENN elements for activation and targeting of Rab27a." in: Journal of cell science, Vol. 132, Issue 9, (2020) (PubMed).

Singh, Mizuno, Wasmeier, Wavre-Shapton, Recchi, Catz, Futter, Tolmachova, Hume, Seabra: "Distinct and opposing roles for Rab27a/Mlph/MyoVa and Rab27b/Munc13-4 in mast cell secretion." in: The FEBS journal, Vol. 280, Issue 3, pp. 892-903, (2013) (PubMed).

Figueiredo, Wasmeier, Tarafder, Ramalho, Baron, Seabra: "Rab3GEP is the non-redundant guanine nucleotide exchange factor for Rab27a in melanocytes." in: The Journal of biological chemistry, Vol. 283, Issue 34, pp. 23209-16, (2008) (PubMed).

Hume, Tarafder, Ramalho, Sviderskaya, Seabra: "A coiled-coil domain of melanophilin is essential for Myosin Va recruitment and melanosome transport in melanocytes." in: Molecular biology of the cell, Vol. 17, Issue 11, pp. 4720-35, (2006) (PubMed).

Details zu Melanophilin

Target: Melanophilin (MLPH)

Andere Bezeichnung: Mlph

Hintergrund: 2210418F23Rik, melanophilin, Mlph, ln, l1Rk3, Slac-2a, D1Wsu84e, l(1)-3Rk, 5031433I09Rik, leaden, AW228792, OTTMUSP00000025451

Gen-ID: 171531, 316620

NCBI Accession: NP_443748