NCF1 Antikörper (C-Term)

Produktnummer ABIN184583

Der Ziege Polyklonal Anti-NCF1-Antikörper wurde für WB, ELISA, IHC, IF und FACS validiert. Er ist geeignet, NCF1 in Proben von Human, Maus und Schwein zu detektieren.

Kurzübersicht für NCF1 Antikörper (C-Term) (ABIN184583)

Target: NCF1 (Neutrophil Cytosol Factor 1 (NCF1))

Reaktivität: Human, Maus, Schwein

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser NCF1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)

Güteklasse: Verified

Produktdetails anti-NCF1 Antikörper

Bindungsspezifität: C-Term

Verwendungszweck: NCF1 / p47phox

Sequenz: SESTKRKLAS AV

Kreuzreaktivität: Rind (Kuh), Human, Maus, Schwein, Ratte

Aufreinigung: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-SESTKRKLASAV, from the C Terminus of the protein sequence according to NP_000256.3.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Immunohistochemistry: Paraffin embedded Human Colon, Neutrophils. Recommended concentration: 5 μg/mL.

Western Blot: Approx 48 kDa band observed in lysates of cell lines Daudi, U251 and U937 and in Mouse Thymus, and approx, 50 kDa in Pig Spleen lysates (calculated MW of 44.7 kDa according to Human NP_000256.4, 46.0 kDa according to Mouse NP_001272966.1 and 45

Peptide ELISA: antibody detection limit dilution 1:20000.

Kommentare:

Immunofluorescence: Strong expression of the protein seen in the cytoplasm of HeLa cells. Recommended concentration: 10μg/ml.

Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concentration: 10ug/ml.<

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Minimize freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Details zu NCF1

Target: NCF1 (Neutrophil Cytosol Factor 1 (NCF1))

Andere Bezeichnung: NCF1

Hintergrund: NCF1, p47phox, FLJ79451, NCF1A, NOXO2, SH3PXD1A, neutrophil cytosolic factor 1, Neutrophil cytosolic factor-1 (47kD), neutrophil cytosolic factor 1 (47kD, chronic granulomatous disease, autosomal 1), NADPH oxidase organizer 2

Gen-ID: 653361, 17969, 114553

NCBI Accession: NP_000256

Pathways: PI3K-Akt Signalweg