Protein A Antikörper

Produktnummer ABIN7477945

Dieses Maus Monoklonal-Antikörper erkennt spezifisch Protein A in ELISA. Er zeigt eine Reaktivität gegenüber Staphylococcus aureus und wurde in 1 Publikation erwähnt.

Kurzübersicht für Protein A Antikörper (ABIN7477945)

Target: Protein A

Reaktivität: Staphylococcus aureus

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Protein A Antikörper ist unkonjugiert

Applikation: ELISA

Klon: 4F9A8

Produktdetails anti-Protein A Antikörper

Spezifität: Protein A Antibody, mAb, Mouse detects natural Protein A, variety of recombinant Protein A variants and MabSelect SuReTM ligand.

Produktmerkmale: Protein A Antibody, mAb, Mouse is produced from the hybridoma resulting from fusion of SP2/0-Ag14 myeloma and B-lymphocytes obtained from mouse immunized with purified recombinant Protein A.

Aufreinigung: Protein A affinity column

Immunogen: purified recombinant Protein A

Isotyp: IgG1 kappa

Anwendungsinformationen

Applikationshinweise: Working concentrations for specific applications should be empirically determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the detection methods, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.

ELISA Capture: 1-10 µg/mL
ELISA Detection: 0. 05 - 0. 2 µg/mL
Recommended antibody pairing for sandwich immunoassay: Capture with A01778 and detected by A01779,
Western Blot: 0. 5 -1 µg/mL
Other applications: user-optimized

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Reconstitute the lyophilized antibody with 200 µl deionized water (or equivalent) to a final antibody concentration of 0.5 mg/mL.

Konzentration: 0.5 mg/mL

Buffer: lyophilized with PBS, pH 7.4, containing 0.02 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Protein A Antibody, mAb, Mouse should be stored lyophilized until use. It remains stable in lyophilized form for at least two years if stored at -20 °C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8 °C or for up to 12 months at -20 °C or below.

Referenzen für anti-Protein A Antikörper (ABIN7477945)

Treise, Huber, Klein-Rodewald, Heinemeyer, Grassmann, Basler, Adler, Rathkolb, Helming, Andres, Klaften, Landbrecht, Wieland, Strom, McCoy, Macpherson, Wolf, Groettrup, Ollert, Neff, Gailus-Durner et al.: "Defective immuno- and thymoproteasome assembly causes severe immunodeficiency. ..." in: Scientific reports, Vol. 8, Issue 1, pp. 5975, (2018) (PubMed).

Details zu Protein A

Target: Protein A

Hintergrund: Protein A, a bacterial cell wall protein isolated from Staphylococcus aureus, binds to mammalian IgGs mainly through Fc regions. The Protein A is most commonly bound to a chromatography resin, used for production of antibody drugs or reagents. Protein A antibody can be used for quantification of Protein A in drugs and foods or for evaluation of Protein A resin. This Protein A mAb is advantageous due to its IgG1 isotype, for mouse IgG1 has weak binding capacity to Protein A