His Tag Antikörper (iFluor 488)

Produktnummer ABIN1842280

Dieses Maus Monoklonal-Antikörper erkennt spezifisch His Tag in . Er zeigt eine Reaktivität gegenüber .

Kurzübersicht für His Tag Antikörper (iFluor 488) (ABIN1842280)

Target: His Tag

Reaktivität: Bitte anfragen

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser His Tag Antikörper ist konjugiert mit iFluor 488

Applikation: Bitte anfragen

Produktdetails anti-His Tag Antikörper (iFluor 488)

Marke: THE™

Sequenz: HHHHHH

Spezifität: THETM His Tag Antibody [iFluor 488], mAb, Mouse recognizes N-terminal, internal and C-terminal His-tagged proteins.

Aufreinigung: Protein A affinity column

Immunogen: A synthetic peptide HHHHHH coupled to KLH

Isotyp: IgG1 kappa

Anwendungsinformationen

Applikationshinweise: Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
Immunofluorescent staining: 1-4 µg/mL
Flow cytometry: 1-4 µg/mL
Other applications: user-optimized

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Reconstitute the lyophilized antibody with deionized water (or equivalent) to a final antibody concentration of 0.5 mg/mL.

Konzentration: 0.5 mg/mL

Buffer: lyophilized with PBS, pH 7.4, containing 10 mg/mL BSA and 0.02 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: The lyophilized product remains stable up to 2 year at -20 °C from date of receipt. Upon reconstitution, it can be stored for 2-3 weeks at 2-8 °C or for up to 12 months at -20 °C or below.

Details zu His Tag

Target: His Tag

Substanzklasse: Tag

Hintergrund: The His tag is a short amino acid sequence consisting of Histidine (His) residues appended to recombinant proteins. Ni-charged resins are used for the purification of His-tagged proteins from eukaryotic and prokaryotic expression systems. An anti-His tag antibody is a useful tool for the analysis of His-tagged proteins.THETM His Tag Antibody [iFluor 488], mAb, Mouse is THETM His Tag Antibody (A00186) conjugated with iFluor 488 under optimal conditions with an F/P ratio of 4-8. It is suitable for detecting the expression level of His-tagged proteins. iFluor 488 is a bright and photostable fluorescent dye. It is an excellent alternative to Alexa Fluor 488.