Notch1 Antikörper (FITC)

Produktnummer ABIN1819821

Dieses Anti-Notch1-Antikörper ist ein Maus Monoklonal-Antikörper zur Detektion von Notch1 in WB, IHC, FACS und IHC (fro). Geeignet für Human und Maus.

Kurzübersicht für Notch1 Antikörper (FITC) (ABIN1819821)

Target: Notch1 (NOTCH1) (Notch 1 (NOTCH1))

Reaktivität: Human, Maus

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Notch1 Antikörper ist konjugiert mit FITC

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro))

Klon: MN1A

Produktdetails anti-Notch1 Antikörper (FITC)

Spezifität: Recognizes Notch 1, one of the four major transmembrane receptors (Notch 1-4) of the Notch signalling pathway, which is activated through binding to DSL domain-containing membrane-bound specific ligands. The Notch signalling pathway is an evolutionarily conserved pathway in multi-cellular organisms, which is vital for cell-cell communication, important during fundamental developmental and physiological processes, including regulation of cell fate decisions during neuronal, cardiac and endocrine development, stem cell haematopoiesis, thymic T-cell development, and both tumor progression and suppression. Ligation of Notch receptors by their specific ligands, Jagged1 (CD339), Jagged2, Delta like-1 (DLL1), DLL3 and DLL4, on physically adjacent signal receiving cells, induces proteolysis of the receptors by ADAM-family metalloproteases and gamma-secretase complex, within the transmembrane domain, releasing the Notch intracellular domain (NICD) to translocate to the nucleus. Subsequent signal transduction then occurs through either the CSL-NICD-Mastermind complex cascade (canonical pathway), or NF-kappaB-NICD and CSL-NICD-Deltex complex signalling cascades (non-canonical pathway). The canonical pathway inhibits the differentiation of stem cells or progenitor cells, whilst the non-canonical pathway promotes differentiation. Notch 1 is expressed in a range of cells including hematopoietic cells in mouse fetal liver, adult thymus and bone marrow. Notch 1 signalling plays a role in follicular differentiation, tissue homeostasis, and in both CD4+ and CD8+ cell maturation in the thymus. Studies have also implicated Notch 1 in the regulation of lymphopoiesis, myelopoiesis, and neurogenesis.

Aufreinigung: Protein G purified

Immunogen: Synthetic peptide corresponding to cdc10-NCR region within mouse Notch1.

Type of Immunogen: Synthetic peptide

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Approved: Flo (1:1 - 1:10), IHC, IHC-Fr, WB

Usage: The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

Kommentare:

Target Species of Antibody: Mouse

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: PBS, 0.09 % sodium azide, 1 % BSA

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: 4°C or -20°C, Avoid freeze-thaw cycles.

Details zu Notch1

Target: Notch1 (NOTCH1) (Notch 1 (NOTCH1))

Andere Bezeichnung: NOTCH1

Hintergrund: Name/Gene ID: NOTCH1

Synonyms: NOTCH1, Notch 1, TAN1

Gen-ID: 4851

UniProt: P46531

Pathways: Notch Signalweg, Stem Cell Maintenance, Regulation of Muscle Cell Differentiation, Tube Formation, Skeletal Muscle Fiber Development