This antibody recognises the alpha chain of the CD8 homologue, a 33-35 kDa cell surface protein. Clone 11-39 recognises all polymorphic forms of chicken CD8 alpha.
Aufreinigung
Affinity chromatography on Protein G
Immunogen
Chicken T-cells. Lymph node cells from immunised Balb/c mice were fused with cells of the SP2/0 myeloma cell line.
Flow Cytometry: Neat - 1/5, use 10 μL of the suggested working dilution to label 10^6 cells in100l. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Rekonstitution
Restore with 1.0 mL distilled water
Konzentration
0.1 mg/mL
Buffer
PBS, pH 7.4 containing 0.09 % Sodium Azide and 1 % Bovine Serum Albumin
Konservierungsmittel
Sodium azide
Vorsichtsmaßnahmen
Use of Sodium Azide as a preservative will substantially inhibit the enzyme activity of horseradish peroxidase.
Handhabung
Avoid repeated freezing and thawing.
Lagerung
4 °C/-20 °C
Informationen zur Lagerung
Store the lyophilized antibody at 2-8 °C. Store the antibody after reconstitution for several weeks at 2-8 °C. Prepare working dilution only prior to immediate use. For extended storage after reconstitution, we suggest the addition of an equal volume of glycerol to make a final glycerol concentration of 50% followed by storage at -20 °C. The concentration of protein and buffer salts will decrease to one-half of the original after the addition of glycerol.
CD8 is expressed as either alpha/alpha homodimers or alpha/beta heterodimers on a subpopulation of T cells and NK cells.Synonyms: CD8B, CD8B1, T-cell surface glycoprotein CD8 beta chain