IL-2 Antikörper

Produktnummer ABIN181831

Dieser Maus Monoklonal Antikörper detektiert spezifisch IL-2 in EIA und Func. Es zeigt Reaktivität gegenüber Proben von Huhn, Avian und Säugetier.

Kurzübersicht für IL-2 Antikörper (ABIN181831)

Target: IL-2 (IL2) (Interleukin 2 (IL2))

Reaktivität: Huhn, Avian, Säugetier

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser IL-2 Antikörper ist unkonjugiert

Applikation: Enzyme Immunoassay (EIA), Functional Studies (Func)

Klon: 4F12

Produktdetails anti-IL-2 Antikörper

Spezifität: This antibody SM1540A recognises natural and recombinant IL-2 expressed in either E.coli or CHO cells. Clone 4F12 neutralises the proliferative activity of rchIL-2 and recognises a different epitope to Clone 10E7 product code (Cat.-No SM1539A). This antibody can be used as the Neutralising antibody for recombinant Chicken IL-2 bioassays (See Product code SA028).

Aufreinigung: Affinity Chromatography on Protein G

Immunogen: Recombinant Chicken IL-2. Remarks: Spleen cells from immunised mice were fused with cells of the mouse Sp2/0-Ag myeloma cell line.

Isotyp: IgG2a

Anwendungsinformationen

Applikationshinweise: ELISA: 5-10g as capture antibody. Western Blot: Non-reducing conditions. Functional Assays: 2-20g/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: PBS without preservatives, None

Konservierungsmittel: Without preservative

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody prior to reconstitution at -20 °C. Following reconstitution the antibody can be stored at 2-8 °C for one month or at -20 °C for longer.

Details zu IL-2

Target: IL-2 (IL2) (Interleukin 2 (IL2))

Andere Bezeichnung: Interleukin-2 / IL2

Hintergrund: Interleukin 2 (IL2) is a secreted cytokine that is important for the proliferation of T and B lymphocytes. The receptor of this cytokine is a heterotrimeric protein complex whose gamma chain is also shared by interleukin 4 (IL4) and interleukin 7 (IL7). The expression of this gene in mature thymocytes is monoallelic, which represents an unusual regulatory mode for controlling the precise expression of a single gene. The targeted disruption of a similar gene in mice leads to ulcerative colitis like disease, which suggests an essential role of this gene in the immune response to antigenic stimuli. IL2 has been shown to have antitumor effects in some studies. This is probably mediated by cytotoxic effector cells.Synonyms: IL-2, T-cell growth factor, TCGF

Gen-ID: 3558

NCBI Accession: NP_000577

UniProt: P60568

Pathways: JAK-STAT Signalweg, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Activated T Cell Proliferation