IL-13 Antikörper (Biotin)

Produktnummer ABIN181766

Der Kaninchen Polyklonal Anti-IL-13-Antikörper wurde für WB und EIA validiert. Er ist geeignet, IL-13 in Proben von Maus zu detektieren.

Kurzübersicht für IL-13 Antikörper (Biotin) (ABIN181766)

Target: IL-13 (IL13) (Interleukin 13 (IL13))

Reaktivität: Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser IL-13 Antikörper ist konjugiert mit Biotin

Applikation: Western Blotting (WB), Enzyme Immunoassay (EIA)

Produktdetails anti-IL-13 Antikörper (Biotin)

Spezifität: This antibody detects Interleukin-13.

Aufreinigung: Affinity chromatography

Immunogen: Highly pure (> 98 %) recombinant murine IL-13

Anwendungsinformationen

Applikationshinweise: ELISA: Direct: To detect mIL-13 (using 100 μL/well antibody solution) a concentration of0.25 - 1.0 μg/mL of this antibody is required. In conjunction with compatible secondaryreagents, it allows the detection of at least 0.2 - 0.4 ng/well of recombinant mIL-13. Sandwich: To detect mIL-13 (using 100 μL/well antibody solution) a concentration of0.25 - 1.0 μg/mL of this antibody is required. In conjunction with Polyclonal Anti-MurineIL-13 as a capture antibody, it allows the detection of at least 0.2 - 0.4 ng/well ofrecombinant mIL-13. Western blot: To detect mIL-13 this antibody can be used at a concentration of

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Rekonstitution: Centrifuge vial prior to opening. Restore in sterile PBS containing 0.1 % BSA to a concentration of 0.1 - 1.0 mg/mL.

Buffer: PBS, pH 7.2

Lagerung: 4 °C

Informationen zur Lagerung: Store the antibody undiluted at 2-8 °C. DO NOT FREEZE! This product is photosensitive and should protected from light.

Details zu IL-13

Target: IL-13 (IL13) (Interleukin 13 (IL13))

Andere Bezeichnung: Interleukin-13 / IL13

Hintergrund: Human IL13 was originally identified by differential screening of an anti CD28 activated human peripheral blood mononuclear cell cDNA library as an induction specific novel cytokine. It was also isolated from cDNA libraries of human T cell clones using the murine IL13 (P600) cDNA as a probe. Human IL13, a pleiotropic cytokine, is produced by activated Th0, Th1 like, Th2 like, and CD8 T cells. The gene for human IL13 maps to chromosome 5 and is closely linked to the genes for IL3, IL4, IL5, and GMCSF. IL13 inhibits proinflammatory cytokine production and stimulates antibody production. It induces proliferation in the human pre myeloid cell line TF1. IL13 has multiple effects on the differentiation and functions of monocytes and macrophages. It suppresses cytotoxic functions and induces changes in the morphology of human monocytes and in the phenotype of human monocytes and B cells by upregulating MHC class II expression. IL13 will also decrease the production of nitric oxide by activated murine macrophages, leading to impaired parasiticidal activity. Human and mouse interleukin 13 share approximately 58 % amino acid sequence identity. Although human and mouse IL13 are equally active on human cells, human IL13 is much less active than mouse IL13 on mouse cells. Human IL13 and human IL4 also share approximately 30 % sequence homology and have similar biological functions.Synonyms: IL-13, NC30

Gen-ID: 16163

NCBI Accession: NP_032381

UniProt: P20109

Pathways: JAK-STAT Signalweg, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Proton Transport