IL12A Antikörper (Biotin)

Produktnummer ABIN181756

Dieses Ziege Polyklonal-Antikörper erkennt spezifisch IL12A in WB und EIA. Er zeigt eine Reaktivität gegenüber Maus.

Kurzübersicht für IL12A Antikörper (Biotin) (ABIN181756)

Target: IL12A (Interleukin 12 alpha (IL12A))

Reaktivität: Maus

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser IL12A Antikörper ist konjugiert mit Biotin

Applikation: Western Blotting (WB), Enzyme Immunoassay (EIA)

Produktdetails anti-IL12A Antikörper (Biotin)

Spezifität: This antibody detects Interleukin-12.

Aufreinigung: Affinity chromatography

Immunogen: Highly pure (> 98 %) recombinant murine IL-12

Anwendungsinformationen

Applikationshinweise: ELISA: Direct: To detect mIL-12 (using 100 μL/well antibody solution) a concentration of0.25 - 1.0 μg/mL of this antibody is required. In conjunction with compatible secondaryreagents, it allows the detection of at least 0.2 - 0.4 ng/well of recombinant mIL-12. Sandwich: To detect mIL-12 (using 100 μL/well antibody solution) a concentration of0.25 - 1.0 μg/mL of this antibody is required. In conjunction with Polyclonal Anti-MurineIL-12 as a capture antibody, it allows the detection of at least 0.2 - 0.4 ng/well ofrecombinant mIL-12. Western blot: To detect mIL-12 this antibody can be used at a concentration of0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detectionlimit for recombinant mIL-12 is 1.5 - 3.0 ng/lane, under either reducing or non-reducingconditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Rekonstitution: Centrifuge vial prior to opening. Restore in sterile PBS containing 0.1 % BSA to a concentration of 0.1 -1 .0 mg/mL.

Buffer: PBS, pH 7.2

Handhabung: This product is photosensitive and should be protected from light

Lagerung: 4 °C

Informationen zur Lagerung: Store the antibody undiluted at 2-8 °C. DO NOT FREEZE!

Details zu IL12A

Target: IL12A (Interleukin 12 alpha (IL12A))

Andere Bezeichnung: Interleukin-12 alpha / IL12A

Hintergrund: Interleukin 12 (IL12) can act as a growth factor for activated T and NK cells, enhance the lytic activity of NK/lymphokine activated killer cells, and stimulate the production of IFN gamma by resting PBMC. IL12 induces IFN gamma, TNF alpha production in T and NK cells, costimulation of PBL proliferation, proliferation/differentiation of TH1 T lymphocytes. It is downregulated by IL10, homodimeric p40 antagonistic to functional p70 heterodimer. IL12R beta 1 binds p40 but when dimeric with IL12R beta 2 binds p35.Synonyms: CLMF p35, Cytotoxic lymphocyte maturation factor 35 kDa subunit, IL-12 subunit p35, IL-12A, NK cell stimulatory factor chain 1, NKSF1

Gen-ID: 16159

NCBI Accession: NP_032377

UniProt: P43431

Pathways: JAK-STAT Signalweg, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Activated T Cell Proliferation