LGALS3
Reaktivität: Human
WB, IF
Wirt: Maus
Polyclonal
unconjugated
Applikationshinweise
ELISAIndirect: To detect hGalectin-3 (using 100 μL/well antibody solution) a concentration of0.5-2.0 μg/mL of this antibody is required. In conjunction with compatible secondaryreagents, allows the detection of at least 0.2-0.4 ng/well of recombinant HumanGalectin-3. Sandwich: To detect Human Galectin-3 (using 100 μL/well antibody solution) aconcentration of 0.5-2.0 μg/mL of this antibody is required. In conjunction withBiotinylated Polyclonal Anti-Human Galectin-3 (ABIN181681 or ABIN181682) as aDetection antibody, it allows the detection of at least 0.2-0.4 ng/well of recombinantHuman Galectin-3. Western blotTo detect Human Galectin-3, this antibody can be used at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for
Beschränkungen
Nur für Forschungszwecke einsetzbar
Rekonstitution
Restore in sterile water to a concentration of 0.1-1.0 mg/mL.
Buffer
PBS, pH 7.2 without preservatives.
Konservierungsmittel
Without preservative
Handhabung
Avoid repeated freezing and thawing.
Lagerung
-20 °C
Informationen zur Lagerung
Store the lyophilized antibody at -20 °C. Following reconstitution it is stable for two weeks at 2-8 °C. Frozen aliquots are stable for 6 months when stored at -20 °C.
Haltbarkeit
6 months
Target
Galectin 3 (LGALS3)
Andere Bezeichnung
Galectin-3
Substanzklasse
Chemical
Hintergrund
Galectins are a new family of animal lectins which appear to exhibit a variety of biological functions. Lectins, of either plant or animal origin, are carbohydrate binding proteins that interact with glycoprotein and glycolipids on the surface of animal cells. The Galectins are lectins that recognize and interact with beta-galactoside moieties. Galectin 3 is one of the more extensively studied members of this family and is a 30 kDa protein. Due to a C-terminal carbohydrate binding site, Galectin 3 is capable of binding IgE and mammalian cell surfaces only when homodimerized or homooligomerized. Galectin 3 is normally distributed in epithelia of many organs, in various inflammatory cells, including macrophages, as well as dendritic cells and Kupffer cells. The expression of this lectin is up-regulated during inflammation, cell proliferation, cell differentiation and through trans-activation by viral proteins.Synonyms: 35 kDa lectin, CBP35, Carbohydrate-binding protein 35, GAL3, GALBP, Galactose-specific lectin 3, IgE-binding protein, L-31, L-34 galactoside-binding lectin, Laminin-binding protein, Lectin L-29, Lgals3, Mac-2