APOE Antikörper (Isoform E3)

Produktnummer ABIN181668

Produktdetails anti-APOE Antikörper

Target: APOE (Apolipoprotein E (APOE))

Bindungsspezifität: Isoform E3

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser APOE Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Enzyme Immunoassay (EIA)

Spezifität: This antibody detects ApoE3. Cross reacts with ApoE2 and ApoE4.

Aufreinigung: Immunoaffinity chromatography

Immunogen: Highly pure (> 98 %) recombinant human ApoE3

Anwendungsinformationen

Applikationshinweise: ELISA: Indirect: To detect hApoE3 (using 100 μL/well antibody solution) a concentration of0.5 - 2.0 μg/mL of this antibody is required. In conjunction with compatible secondaryreagents, it allows the detection of at least 0.2 - 0.4 ng/well of recombinant hApoE3. Sandwich: To detect hApoE3 (using 100 μL/well antibody solution) a concentration of0.5 - 2.0 μg/mL of this antibody is required. In conjunction with Biotinylated Anti-HumanApoE3 as a detection antibody, it allows the detection of at least 0.2 - 0.4 ng/well ofrecombinant hApoE3. Western blot: To detect hApoE3 this antibody can be used at a concentration of0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detectionlimit for recombinant hApoE3 is 1.5 - 3.0 ng/lane, under either reducing or non-reducingconditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Rekonstitution: Centrifuge vial prior to opening. Restore in sterile water to a concentration of 0.1 - 1.0 mg/mL.

Buffer: PBS, pH 7.2

Handhabung: Avoid repeated freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Store the lyophilized antibody at -20 °C. Following reconstitution it is stable for two weeks at 2 - 8 °C. Frozen aliquots are stable for 6 months when stored at -20 °C.

Haltbarkeit: 6 months

Details zu APOE

Target: APOE (Apolipoprotein E (APOE))

Andere Bezeichnung: Apolipoprotein E (Apo E) (APOE Produkte)

Synonyme: ad2 antikoerper, apoprotein antikoerper, im:7036787 antikoerper, wu:fb69a05 antikoerper, zgc:110064 antikoerper, apoe antikoerper, AI255918 antikoerper, AD2 antikoerper, LDLCQ5 antikoerper, LPG antikoerper, APOEA antikoerper, Apo-E antikoerper, apolipoprotein E antikoerper, apolipoprotein Ea antikoerper, apoe antikoerper, apoea antikoerper, Apoe antikoerper, APOE antikoerper

Hintergrund: Apolipoprotein E is essential for the normal catabolism of triglyceride rich lipoprotein constituents. The apolipoprotein E gene is mapped to chromosome 19 in a cluster with APOC1 and APOC2. Defects in Apolipoprotein E result in familial dysbetalipoproteinemia, or type III hyperlipoproteinemia (HLP III), in which increased plasma cholesterol and triglycerides are the consequence of impaired clearance of chylomicron and VLDL remnants. Mutations in the APOE gene confer susceptibility to Alzheimer's disease by affecting amyloid-beta deposition.Synonyms: Apo-E, ApoE

Gen-ID: 348

NCBI Accession: NP_000032

UniProt: P02649

Pathways: Regulation of Cell Size, Lipid Metabolism