APOE Antikörper (Isoform E3) (Biotin)

Produktnummer ABIN181665

Dieses Anti-APOE-Antikörper ist ein Kaninchen Polyklonal-Antikörper zur Detektion von APOE in WB und EIA. Geeignet für Human.

Kurzübersicht für APOE Antikörper (Isoform E3) (Biotin) (ABIN181665)

Target: APOE (Apolipoprotein E (APOE))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser APOE Antikörper ist konjugiert mit Biotin

Applikation: Western Blotting (WB), Enzyme Immunoassay (EIA)

Produktdetails anti-APOE Antikörper (Biotin)

Bindungsspezifität: Isoform E3

Spezifität: This antibody detects ApoE3. Cross reacts with Human ApoE2 and Human ApoE4 in both Sandwich ELISA and Western Blot.

Aufreinigung: Affinity Chromatography.

Immunogen: E.Coli derived recombinant Human ApoE3.

Anwendungsinformationen

Applikationshinweise: Direct ELISA: To detect Human ApoE3 by Direct ELISA (using 100 μL/well antibody solution)a concentration of approx. 1.0 μg/mL of this antibody is required. This Biotinylated polyclonalantibody allows the detection of at least 0.2-0.4 ng/well of recombinant Human ApoE3. Sandwich ELISA: To detect Human ApoE3 by Sandwich ELISA (using 100 μL/well) aconcentration of 0.25-1.0 μg/mL of this antibody is required. This Biotinylated antibody, inconjunction with Affinity purified anti-Human ApoE3 (ABIN181667 or ABIN181668) as acapture antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant HumanApoE3. Western blot: To detect Human ApoE3 this antibody can be used at a concentration of0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limitfor recombinant Human ApoE3 is 1.5-3.0 ng/lane, under either reducing or non-reducingconditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Rekonstitution: Restore in sterile PBS containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL.

Buffer: PBS, pH 7.2 without preservatives.

Konservierungsmittel: Without preservative

Handhabung: Avoid repeated freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Store the lyophilized antibody at -20 °C. Following reconstitution it is stable for two weeks at 2-8 °C. Frozen aliquots are stable for 6 months when stored at -20 °C.

Haltbarkeit: 6 months

Details zu APOE

Target: APOE (Apolipoprotein E (APOE))

Andere Bezeichnung: Apolipoprotein E (Apo E)

Hintergrund: Apolipoprotein E3 mediates the binding, internalization, and catabolism of lipoprotein particles. It can serve as a ligand for the LDL (apo B/E) receptor and for the specific apo E receptor (chylomicron remnant) of hepatic tissues.Synonyms: Apo-E, ApoE

Gen-ID: 348

NCBI Accession: NP_000032

UniProt: P02649

Pathways: Regulation of Cell Size, Lipid Metabolism