Nanog Antikörper (Biotin)

Produktnummer ABIN181657

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch Nanog in WB und EIA. Er zeigt eine Reaktivität gegenüber Human.

Kurzübersicht für Nanog Antikörper (Biotin) (ABIN181657)

Target: Nanog (NANOG) (Nanog Homeobox (NANOG))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Nanog Antikörper ist konjugiert mit Biotin

Applikation: Western Blotting (WB), Enzyme Immunoassay (EIA)

Produktdetails anti-Nanog Antikörper (Biotin)

Spezifität: This antibody recognizes Human Nanog. Other species not tested.

Aufreinigung: Affinity Chromatography

Immunogen: Highly pure (> 98%) E.coli recombinant Human Nanog

Anwendungsinformationen

Applikationshinweise: ELISA: Direct: To detect hNanog (using 100 μL/well antibody solution) a concentration of0.25 - 1.0 μg/mL of this antibody is required. In conjunction with compatible secondaryreagents, it allows the detection of at least 0.2 - 0.4 ng/well of recombinant hNanog. Sandwich: To detect hNanog (using 100 μL/well antibody solution) a concentration of0.25 - 1.0 μg/mL of this antibody is required. In conjunction with Polyclonal Anti-HumanNanog as a capture antibody, it allows the detection of at least 0.2 - 0.4 ng/well ofrecombinant hNanog. Western blot: To detect hNanog this antibody can be used at a concentration of0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detectionlimit for recombinant hNanog is 1.5 - 3.0 ng/lane, under either reducing or non-reducingconditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Rekonstitution: Centrifuge vial prior to opening. Restore in sterile PBS containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL.

Buffer: PBS, pH 7.2

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Prior to reconstitution store at 2-8 °C. Following reconstitution store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Details zu Nanog

Target: Nanog (NANOG) (Nanog Homeobox (NANOG))

Andere Bezeichnung: NANOG

Hintergrund: Nanog is a newly identified homeodomain-bearing transcriptional factor. Nanog expression is specific to early embryos and pluripotential stem cells including mouse and human embryonic stem (ES) and embryonic germ (EG) cells. It is a key molecule involved in the signaling pathway for maintaining the capacity for self-renewal and pluripotency, bypassing regulation by the STAT3 pathway. Nanog mRNA is present in pluripotent mouse and human cell lines, and absent from differentiated cells. Nanog-deficient ES cells lose pluripotency and differentiate into extraembryonic endoderm lineage. Thus it is one of the molecular markers suitable for recognizing the undifferentiated state of stem cells in the mouse and human. NANOG is a new marker for testicular carcinoma in situ and germ cell tumors.Synonyms: Homeobox protein NANOG

Gen-ID: 100293888

NCBI Accession: NP_079141

UniProt: Q9H9S0

Pathways: Stem Cell Maintenance