WISP2 Antikörper (Biotin)

Produktnummer ABIN181621

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch WISP2 in WB und EIA. Er zeigt eine Reaktivität gegenüber Human.

Kurzübersicht für WISP2 Antikörper (Biotin) (ABIN181621)

Target: WISP2 (WNT1 Inducible Signaling Pathway Protein 2 (WISP2))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser WISP2 Antikörper ist konjugiert mit Biotin

Applikation: Western Blotting (WB), Enzyme Immunoassay (EIA)

Produktdetails anti-WISP2 Antikörper (Biotin)

Spezifität: This antibody detects Connective Tissue Growth Factor-Like protein.

Aufreinigung: Affinity chromatography

Immunogen: Highly pure (> 98 %) recombinant human CTGFL/WISP-2

Anwendungsinformationen

Applikationshinweise: ELISA: Direct: To detect hCTGFL/WISP-2 by direct ELISA (using 100 μL/well antibodysolution) a concentration of 0.25 - 1.0 μg/mL of this antibody is required. In conjunctionwith compatible secondary reagents, it allows the detection of at least 0.2 - 0.4 ng/well ofrecombinant hCTGFL/WISP-2. Sandwich: To detect hCTGFL/WISP-2 by sandwich ELISA (using 100 μL/well antibodysolution) a concentration of 0.25 - 1.0 μg/mL of this antibody is required. In conjunctionwith Polyclonal Anti-Human CTGFL/WISP-2 as a capture antibody, it allows the detection ofat least 0.2 - 0.4 ng/well of recombinant hCTGFL/WISP-2. Western Blot: To detect hCTGFL/WISP-2 by Western Blot analysis this antibody can be usedat a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondaryreagents the detection limit for recombinant hCTGFL/WISP-2 is 1.5 - 3.0 ng/lane, undereither reducing or non-reducing conditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Rekonstitution: Centrifuge vial prior to opening. Restore in sterile PBS containing 0.1 % BSA to a concentration of 0.1 - 1.0 mg/mL.

Buffer: PBS, pH 7.2

Handhabung: Avoid repeated freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Store the lyophilized antibody at -20 °C. Following reconstitution it is stable for two weeks at 2 - 8 °C. Frozen aliquots are stable for 6 months when stored at -20 °C.

Haltbarkeit: 6 months

Details zu WISP2

Target: WISP2 (WNT1 Inducible Signaling Pathway Protein 2 (WISP2))

Andere Bezeichnung: WISP2

Hintergrund: Synonyms: CCN5, CT58, CTGF-L, CTGFL, Connective tissue growth factor-like protein, Connective tissue growth factor-related protein 58, UNQ228/PRO261, WISP-2, WNT1-inducible-signaling pathway protein 2

Gen-ID: 8839

NCBI Accession: NP_003872

UniProt: O76076

Pathways: WNT Signalweg, Growth Factor Binding