CLCF1 Antikörper

Produktnummer ABIN181612

Der Kaninchen Polyklonal anti-CLCF1 Antikörper (ABIN181612) detektiert spezifisch CLCF1 in WB und EIA. Dieser Antikörper reagiert spezifisch mit Proben aus Human.

Kurzübersicht für CLCF1 Antikörper (ABIN181612)

Target: CLCF1 (Cardiotrophin-Like Cytokine Factor 1 (CLCF1))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser CLCF1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Enzyme Immunoassay (EIA)

Produktdetails anti-CLCF1 Antikörper

Spezifität: This antibody detects NNT-1/BCSF-3.

Aufreinigung: Immunoaffinity chromatography

Immunogen: Highly pure (> 98 %) recombinant human NNT-1/BCSF-3

Anwendungsinformationen

Applikationshinweise: ELISA: Indirect: To detect hNNT-1/BCSF-3 by indirect ELISA (using 100 μL/well antibodysolution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. In conjunction withcompatible secondary reagents, it allows the detection of at least 0.2 - 0.4 ng/well ofrecombinant hNNT-1/BCSF-3. Sandwich: To detect hNNT-1/BCSF-3 by sandwich ELISA (using 100 μL/well antibodysolution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. In conjunction withBiotinylated Anti-Human NNT-1/BCSF-3 as a detection antibody, it allows the detection ofat least 0.2 - 0.4 ng/well of recombinant hNNT-1/BCSF-3. Western Blot: To detect hNNT-1/BCSF-3 by Western Blot analysis this antibody can be usedat a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondaryreagents the detection limit for recombinant hNNT-1/BCSF-3 is 1.5 - 3.0 ng/lane, undereither reducing or non-reducing conditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Rekonstitution: Centrifuge vial prior to opening. Restore in sterile water to a concentration of 0.1 - 1.0 mg/mL.

Buffer: PBS, pH 7.2

Handhabung: Avoid repeated freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Store the lyophilized antibody at -20 °C. Following reconstitution it is stable for two weeks at 2 - 8 °C. Frozen aliquots are stable for 6 months when stored at -20 °C.

Haltbarkeit: 6 months

Details zu CLCF1

Target: CLCF1 (Cardiotrophin-Like Cytokine Factor 1 (CLCF1))

Andere Bezeichnung: CLCF1

Hintergrund: NNT1 belongs to the interleukin 6 family of cytokines, which are involved in cell signaling through phosphorylation of gp130. IL6 family members share similarity in gene structure and have a 4 helix bundle in their protein structure. NNT1 has a sequence of 225-amino acids with a 27-aa signal peptide, a molecular mass of 22 kDa in mature form, and the highest homology to cardiotrophin-1 and ciliary neurotrophic factor. NNT1 may act as a neuroimmunoendocrine modulator of hypothalamus-pituitary-adrenal axis stress response.Synonyms: B-cell-stimulating factor 3, BSF3, CLC, Cardiotrophin-like cytokine factor 1, NNT1, Novel neurotrophin-1

Gen-ID: 23529

NCBI Accession: NP_037378

UniProt: Q9UBD9

Pathways: Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response