WNT3A Antikörper (Biotin)

Produktnummer ABIN181373

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch WNT3A in WB und EIA. Er zeigt eine Reaktivität gegenüber Human.

Kurzübersicht für WNT3A Antikörper (Biotin) (ABIN181373)

Target: WNT3A (Wingless-Type MMTV Integration Site Family, Member 3A (WNT3A))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser WNT3A Antikörper ist konjugiert mit Biotin

Applikation: Western Blotting (WB), Enzyme Immunoassay (EIA)

Produktdetails anti-WNT3A Antikörper (Biotin)

Spezifität: This antibody detects Human WNT-3a. Other species not tested.

Aufreinigung: Affinity Chromatography

Immunogen: E.Coli derived recombinant Human WNT-3a

Anwendungsinformationen

Applikationshinweise: ELISA: Direct: To detect hWNT-3a by direct ELISA (using 100 μL/well antibody solution) aconcentration of 0.25 - 1.0 μg/mL is required. This biotinylated polyclonal antibody, inconjunction with compatible secondary reagents, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hWNT-3a. Sandwich: To detect hWNT-3a by sandwich ELISA (using 100 μL/well antibody solution) aconcentration of 0.25 - 1.0 μg/mL is required. This biotinylated polyclonal antibody, inconjunction with Polyclonal Anti-Human WNT-3a as a capture antibody, allows thedetection of at least 0.2 - 0.4 ng/well of recombinant hWNT-3a. Western Blot: To detect hWNT-3a by Western Blot analysis this antibody can be used at aconcentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagentsthe detection limit for recombinant hWNT-3a is 1.5 - 3.0 ng/lane, under either reducing ornon-reducing conditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Rekonstitution: Restore in sterile PBS containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL.

Buffer: PBS, pH 7.2

Handhabung: Avoid repeated freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Store the lyophilized antibody at -20 °C. Following reconstitution it is stable for two weeks at 2-8 °C. Frozen aliquots are stable for 6 months when stored at -20 °C.

Haltbarkeit: 6 months

Details zu WNT3A

Target: WNT3A (Wingless-Type MMTV Integration Site Family, Member 3A (WNT3A))

Andere Bezeichnung: WNT3A

Hintergrund: Synonyms: Protein Wnt-3a

Gen-ID: 89780

NCBI Accession: NP_149122

UniProt: P56704

Pathways: WNT Signalweg, Regulation of Muscle Cell Differentiation, Regulation of Cell Size, Positive Regulation of Endopeptidase Activity