IL12A Antikörper

Produktnummer ABIN181145

Der Maus Monoklonal anti-IL12A Antikörper wird verwendet zum Nachweis von IL12A in Proben von Human. Er wurde validiert für WB, EIA und IF.

Kurzübersicht für IL12A Antikörper (ABIN181145)

Target: IL12A (Interleukin 12 alpha (IL12A))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser IL12A Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Enzyme Immunoassay (EIA), Immunofluorescence (IF)

Produktdetails anti-IL12A Antikörper

Spezifität: This antibody detects Human Interleukin-12. Other species not tested.

Produktmerkmale: Synonyms: IL-12A, IL-12 subunit p35, NKSF1, CLMF p35, Cytotoxic lymphocyte maturation factor 35 kDasubunit, NK cell stimulatory factor chain 1

Aufreinigung: From Ascites by Affinity Chromatography on Protein A

Immunogen: Highly pure (>98%) recombinant Human IL-12

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: ELISA: In a sandwich ELISA (assuming 100 μL/well), a concentration of 2.0-4.0 μg/mL of thisantibody will detect at least 1000 pg/mL of recombinant Human IL-12 when used with Biotinanti-Human IL-12 antibody (cat. PP1102B) as the detection antibody at a concentration ofapproximately 4.0-8.0 μg/mL. Western Blot: To detect Human IL-12 by Western Blot analysis this antibody can be used ata concentration of 0.50-1.0 μg/mL. Used in conjunction with compatible secondaryreagents the detection limit for recombinant hIL-12 is 0.5-1.0 ng/lane, under non-reducingconditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Rekonstitution: Restore in sterile water to a concentration of 1.0 mg/mL.

Buffer: PBS without preservatives

Konservierungsmittel: Without preservative

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: The lyophilized antibody is stable at room temperature for one month or at -20 °C forlonger. Following reconstitution it is stable at 2-8 °C for six weeks. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.

Haltbarkeit: 12 months

Details zu IL12A

Target: IL12A (Interleukin 12 alpha (IL12A))

Andere Bezeichnung: Interleukin-12 alpha / IL12A

Hintergrund: IL-12 is a potent regulator of cell mediated immune responses and it induces IFN-γ production by NK and T cells. It is produced by activated monocytes/macrophage cells, B lymphocytes and connective tissue type mast cells. Among its biological activities IL-12 promotes the growth and activity of activated NK, CD4+ and CD8+ cells and induces the development of IFN-Gamma producing Th1 cells.Synonyms: CLMF p35, Cytotoxic lymphocyte maturation factor 35 kDa subunit, IL-12 subunit p35, IL-12A, NK cell stimulatory factor chain 1, NKSF1

Gen-ID: 3592

UniProt: P29459

Pathways: JAK-STAT Signalweg, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Activated T Cell Proliferation