MICA Antikörper

Produktnummer ABIN180911

Der Maus Monoklonal anti-MICA Antikörper wird verwendet zum Nachweis von MICA in Proben von Human. Er wurde validiert für FACS, IP, IHC (fro) und Func.

Kurzübersicht für MICA Antikörper (ABIN180911)

Target: MICA (MHC Class I Polypeptide-Related Sequence A (MICA))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser MICA Antikörper ist unkonjugiert

Applikation: Flow Cytometry (FACS), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro)), Functional Studies (Func)

Klon: 6D4

Produktdetails anti-MICA Antikörper

Spezifität: This antibody is specific for nonclassical MHC class I chain A (MICA) and nonclassical MHC class I chain MICB (MICB).

Kreuzreaktivität (Details): Also reacts to: MICB

Produktmerkmale: Synonyms: MHC class I polypeptide-related sequence A, MIC-A, PERB11.1

Aufreinigung: Affinity chromatography on Protein G

Endotoxin-Niveau: Low Endotoxin

Immunogen: MICA transfected C1R cells. Spleen cells from immunised RBF/DnJ mice were fused withcells of the P3 mouse myeloma cell line.

Isotyp: IgG2a

Anwendungsinformationen

Applikationshinweise: Immunoprecipitation. Functional Assays. Flow Cytometry: use 10 μL of the suggested working dilution to label 1x10^6 cells in 100 μL. Immunohistochemistry on frozen sections: 1/100.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: PBS, pH 7.4

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.

Haltbarkeit: 12 months

Details zu MICA

Target: MICA (MHC Class I Polypeptide-Related Sequence A (MICA))

Andere Bezeichnung: MICA

Hintergrund: MICA and MICB are stress inducible antigens, which are closely related and appear functionally indistinguishable. MICA and MICB are ligands for NKG2D, an activating receptor on most natural killer (NK) cells, CD8 T cells and gamma delta T cells. MICA is principally expressed on intestinal epithelium, and several epithelial tumours. Expression may be induced to high surface levels by heat shock, oxidative stress, and virus infection. Clone 6D4 is reported to inhibit the cytotoxicity of NK cells stimulated by IFN alpha-treated dendritic cells.Synonyms: MHC class I polypeptide-related sequence A, MIC-A, PERB11.1

Gen-ID: 4276

UniProt: Q29983

Pathways: Activation of Innate immune Response, Transition Metal Ion Homeostasis, Human Leukocyte Antigen (HLA) in Adaptive Immune Response