Thrombospondin 1 Antikörper (N-Term)

Produktnummer ABIN1781811

Dieses Anti-Thrombospondin 1-Antikörper ist ein Ziege Polyklonal-Antikörper zur Detektion von Thrombospondin 1 in WB, IHC, ELISA, IF und FACS. Geeignet für Human und Maus.

Kurzübersicht für Thrombospondin 1 Antikörper (N-Term) (ABIN1781811)

Target: Thrombospondin 1 (THBS1)

Reaktivität: Human, Maus

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser Thrombospondin 1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS)

Güteklasse: Verified

Produktdetails anti-Thrombospondin 1 Antikörper

Bindungsspezifität: N-Term

Verwendungszweck: Thrombospondin

Sequenz: NRIPESGGDN SVFD

Kreuzreaktivität: Rind (Kuh), Human, Maus, Ratte

Aufreinigung: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Immunohistochemistry: Paraffin embedded Human Spleen. Recommended concentration: 5 μg/mL.

Western Blot: Approx 150 kDa band observed in Mouse Kidney lysates (calculated MW of 129 kDa according to NP_035710.2). Recommended concentration: 0.3-1 μg/mL. Primary incubation 1 hour at room temperature.

Peptide ELISA: antibody detection limit dilution 1:128000.

Kommentare:

Immunofluorescence: Strong expression of the protein seen in the cytoplasm of HeLa and HepG2 cells. Recommended concentration: 10μg/ml.

Flow Cytometry: Flow cytometric analysis of A431 cells. Recommended concentration

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Minimize freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Details zu Thrombospondin 1

Target: Thrombospondin 1 (THBS1)

Andere Bezeichnung: THBS1

Hintergrund: THBS1, thrombospondin 1, THBS, TSP, TSP1, thrombospondin-1p180

Gen-ID: 7057

NCBI Accession: NP_003237

Pathways: Autophagie