TUBB3 Antikörper (AA 443-450)

Produktnummer ABIN1742553

Dieser Anti-TUBB3 Antikörper ist ein Kaninchen Polyklonal Antikörper zur Detektion von TUBB3 in WB, IHC, IP und ICC. Geeignet für Human, Maus und Ratte. Unabhängig validiert für die Verwendung in Western Blotting. Dieses Primary Antibody wurde in 1 Publikation zitiert.

Kurzübersicht für TUBB3 Antikörper (AA 443-450) (ABIN1742553)

Target: TUBB3 (Tubulin, beta 3 (TUBB3))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser TUBB3 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Produktdetails anti-TUBB3 Antikörper

Bindungsspezifität: AA 443-450

Spezifität: Specific for beta3-tubulin.

Immunogen: Synthetic peptide (aa 443-450 of mouse beta3-tubulin) coupled to key-hole limpet hemocyanin via an added N-terminal cysteine.

Anwendungsinformationen

Applikationshinweise: WB: 1:1000 up to 1:10000, ICC: 1:1000 up to 1:5000, IHC: 1:200 up to 1:500, IHC-P: 1:2000 up to 1:4000,

Beschränkungen: Nur für Forschungszwecke einsetzbar

Independent Validation (WB)

Validierung #029822 (Western Blotting)

by: Kinexus Bioinformatics Corporation

No.: #029822

Datum: 28.10.2014

Chargennummer: 302302/3

Validierte Anwendung: Western Blotting

Positivkontrolle: Mouse brain

Negativkontrolle: Mouse heart, HeLa cells, A431 cells

Bewertung: A band was observed in the positive controls at the expected size (~50 kDa), which is lower in the negative controls.

Validierungsbilder

Figure 1: Western Blot for Tubulin, Beta, 3 (TUBB3). Grey arrowhead indicates the expected molecular weight of ~50 kDa.

Protokoll

Primärantikörper:

• Antigen: Tubulin, Beta, 3 (TUBB3) (AA 443-450)
• Catalog number: ABIN1742553
• Lot number: 302302/3
• Dilution: 1:1000

Sekundärantikörper:

• Antibody: Donkey anti-Rabbit IgG Antibody (HRP)
• Lot number: F0613
• Dilution: 1:10,000

Full Protocol:

• 1. Cell/tissue total protein lysates were boiled in 1X SDS Sample Buffer containing 1% SDS and 1.25% β-mercaptoethanol at 95°C for 5 minutes prior to loading.
• 2. 15 μg of boiled lysate were loaded and resolved on a 12% SDS-polyacrylamide gel.
• 3. The Precision Plus Protein™ All Blue Prestained Standards from BioRad (161-0373) were used as molecular mass markers.
• 4. Proteins were transferred onto nitrocellulose membrane by tank transfer and protein transfer was confirmed with Ponceau S staining.
• 5. The immunoblot membrane was blocked in 2.5% skim milk and 1.5% BSA solution in TTBS at room temperature for 60 minutes.
• 6. The membrane was washed in TTBS twice for 5 minutes each.
• 7. The membrane was immersed with the protein side up in the antibody solution in TBS and incubated overnight at 4°C with gentle agitation.
• 8. The membrane was rinsed twice with TTBS.
• 9. The membrane was washed in TTBS twice for 5 minutes each.
• 10. The membrane was washed in TTBS once for 15 minutes.
• 11. The membrane was incubated in the HRP-conjugated secondary antibody solution in TBS for 60 minutes at room temperature with gentle agitation.
• 12. The membrane was rinsed twice with TTBS.
• 13. The membrane was washed in TTBS twice for 5 minutes each.
• 14. The membrane was washed in TTBS once for 15 minutes.
• 15. Signals were detected by chemiluminescence (ECL). The blot was scanned for 320 seconds.
• 16. The membrane was rinsed three times with TTBS.
• 17. Repeated Steps 4-15 with the loading control antibody and its matching secondary antibody. The blot was scanned for 160 seconds.

Anmerkungen: - No experimental challenges noted.

Handhabung

Format: Lyophilized

Rekonstitution: For reconstitution add 200µl H2O.

Konservierungsmittel: Azide and BSA free

Handhabung: Do not freeze! Antibodies should be stored at +4°C when still lyophilized.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Reconstitute and aliquot and store at -20°C until use. Antibodies should be stored at +4°C when still lyophilized.

Referenzen für anti-TUBB3 Antikörper (ABIN1742553)

Kaczor, Rakus, Mozrzymas: "Neuron-astrocyte interaction enhance GABAergic synaptic transmission in a manner dependent on key metabolic enzymes." in: Frontiers in cellular neuroscience, Vol. 9, pp. 120, (2015) (PubMed).

Details zu TUBB3

Target: TUBB3 (Tubulin, beta 3 (TUBB3))

Andere Bezeichnung: beta3-Tubulin (TUBB3)

Hintergrund: Synonyms: TuJ1

Pathways: Microtubule Dynamics, M Phase