alpha Tubulin Antikörper (AA 443-449)

Produktnummer ABIN1742552

Der Ratte Monoklonal Anti-alpha Tubulin-Antikörper wurde für WB, IHC und ICC validiert. Er ist geeignet, alpha Tubulin in Proben von Huhn und Säugetier zu detektieren. Es ist 1 Publikation verfügbar.

Kurzübersicht für alpha Tubulin Antikörper (AA 443-449) (ABIN1742552)

Target: alpha Tubulin (TUBA1)

Reaktivität: Huhn, Säugetier

Wirt: Ratte

Klonalität: Monoklonal

Konjugat: Dieser alpha Tubulin Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC)

Klon: 37B5

Produktdetails anti-alpha Tubulin Antikörper

Bindungsspezifität: AA 443-449

Spezifität: Specific for alpha-tubulin.

Aufreinigung: purified IgG. Azide was added before lyophilization.

Immunogen: Synthetic peptide EGEEEGE (aa 443-449 of rat alpha-tubulin) coupled to key-hole limpet hemocyanin via an added N -terminal cysteine residue.

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: WB: 1 : 1000 up to 1 : 5000 (AP staining)
IP: not tested yet
ICC: 1 : 200 up to 1 : 500
IHC: 1 : 400

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: For reconstitution add 100 µL H2O to get a 1mg/ml solution of antibody in PBS. Then aliquot and store at -20 °C until use.

Buffer: PBS, 0.02% sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Do not store diluted antibody solutions unless you add detergent or carrier proteins such as goat serum, BSA or others. IgG sticks to glass and plastic. Any IgG solution below 0.1 mg/mL protein will quickly adsorb and denature and thus loose activity! Repetitive freeze-thawing of dilute purified IgG is almost certain to lead to substantial losses.

Lagerung: -20 °C

Informationen zur Lagerung: Unlabeled antibodies are stable in this form without loss of quality at ambient temperatures for several weeks or even months. They can be stored at 4 °C for several years.

Referenzen für anti-alpha Tubulin Antikörper (ABIN1742552)

Luchkina, Huupponen, Clarke, Coleman, Keinänen, Taira, Lauri: "Developmental switch in the kinase dependency of long-term potentiation depends on expression of GluA4 subunit-containing AMPA receptors." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 111, Issue 11, pp. 4321-6, (2014) (PubMed).

Details zu alpha Tubulin

Target: alpha Tubulin (TUBA1)

Andere Bezeichnung: alpha-Tubulin

Pathways: Microtubule Dynamics