alpha Tubulin Antikörper (AA 443-449)

Produktnummer ABIN1742550

Dieses Anti-alpha Tubulin-Antikörper ist ein Kaninchen Polyklonal-Antikörper zur Detektion von alpha Tubulin in WB, ELISA, IHC und ICC. Geeignet für Human, Maus, Ratte, Huhn und Säugetier. Dieses Primary Antibody wurde in 1 Publikation zitiert.

Kurzübersicht für alpha Tubulin Antikörper (AA 443-449) (ABIN1742550)

Target: alpha Tubulin (TUBA1)

Reaktivität: Human, Maus, Ratte, Huhn, Säugetier

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser alpha Tubulin Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunocytochemistry (ICC)

Produktdetails anti-alpha Tubulin Antikörper

Bindungsspezifität: AA 443-449

Spezifität: Specific for alpha-tubulin (glu- and tyr-alpha-tubulin).

Aufreinigung: Affinity purified with the immunogen. Rabbit serum albumin was added for stabilization.

Immunogen: Synthetic peptide EGEEEGE (aa 443-449 of rat alpha-tubulin) coupled to key-hole limpet hemocyanin via an added N-terminal cysteine.

Anwendungsinformationen

Applikationshinweise: WB: 1 : 1000 up to 1 : 5000 (AP staining)
IP: not tested yet
ICC: 1 : 100 up to 1 : 1000
IHC: 1 : 400

Kommentare:

ELISA: Suitable as detector antibody for sandwich-ELISA with ABIN1742547 or ABIN1742549 as capture antibodies (protocol for sandwich-ELISA).

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: For reconstitution add 100 µL H2O to get a 1mg/ml solution of antibody in PBS. Then aliquot and store at -20 °C until use.

Buffer: PBS

Handhabung: Affinity purified antibodies are less robust than antisera, since protease inhibitors are also removed during purification. Hence, storage at 4 °C for prolonged periods (i.e. several weeks), is not recommended.

Lagerung: -20 °C

Informationen zur Lagerung: Unlabeled lyophilized antibodies are stable in this form without loss of quality at ambient temperatures for several weeks or even months. They can be stored at 4°C for several years. Lyophilized antibodies must not be stored in the freezer, they may be destroyed!

Referenzen für anti-alpha Tubulin Antikörper (ABIN1742550)

Wilhelm, Mandad, Truckenbrodt, Kröhnert, Schäfer, Rammner, Koo, Claßen, Krauss, Haucke, Urlaub, Rizzoli: "Composition of isolated synaptic boutons reveals the amounts of vesicle trafficking proteins." in: Science (New York, N.Y.), Vol. 344, Issue 6187, pp. 1023-8, (2014) (PubMed).

Details zu alpha Tubulin

Target: alpha Tubulin (TUBA1)

Andere Bezeichnung: alpha-Tubulin

Pathways: Microtubule Dynamics