Telefon:
+49 (0)241 95 163 153
Fax:
+49 (0)241 95 163 155
E-Mail:
orders@antikoerper-online.de

beta Actin Antikörper (AA 2-16)

Dieses Anti-beta Actin-Antikörper ist ein Kaninchen Polyklonal-Antikörper zur Detektion von beta Actin in WB, IHC und ICC. Geeignet für Maus, Ratte, Huhn und Zebrafisch (Danio rerio). Unabhängig validiert für die Verwendung in Western Blotting. Dieses Primary Antibody wurde in 3+ Publikationen zitiert.
Produktnummer ABIN1742508

Kurzübersicht für beta Actin Antikörper (AA 2-16) (ABIN1742508)

Target

Alle beta Actin (ACTB) Antikörper anzeigen
beta Actin (ACTB) (Actin, beta (ACTB))

Reaktivität

  • 199
  • 138
  • 116
  • 55
  • 45
  • 42
  • 40
  • 27
  • 27
  • 24
  • 17
  • 15
  • 11
  • 10
  • 7
  • 7
  • 6
  • 6
  • 5
  • 4
  • 3
  • 3
  • 2
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
Maus, Ratte, Huhn, Zebrafisch (Danio rerio)

Wirt

  • 117
  • 103
  • 5
  • 1
  • 1
Kaninchen

Klonalität

  • 114
  • 111
Polyklonal

Konjugat

  • 161
  • 11
  • 11
  • 9
  • 3
  • 3
  • 3
  • 3
  • 3
  • 3
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
Dieser beta Actin Antikörper ist unkonjugiert

Applikation

  • 191
  • 79
  • 77
  • 47
  • 46
  • 44
  • 32
  • 27
  • 13
  • 13
  • 7
  • 6
  • 6
  • 6
  • 5
  • 2
  • 1
  • 1
  • 1
  • 1
Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC)
  • Bindungsspezifität

    • 41
    • 17
    • 16
    • 16
    • 8
    • 7
    • 3
    • 3
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 2-16

    Kreuzreaktivität (Details)

    May cross-react to alpha- and gamma-actin due to sequence homology.

    Aufreinigung

    Affinity purified with the immunogen. Rabbit serum albumin was added for stabilization.

    Immunogen

    Synthetic peptide (aa 2-16 of mouse beta-actin) coupled to KLH via a C-terminally added cysteine.
  • Applikationshinweise

    WB: 1 : 1000 up to 1 : 5000
    IP: not tested yet
    ICC: 1 : 200 up to 1 : 500
    IHC: 1 : 200

    Kommentare

    ICC: Methanol fixation is recommended.

    Beschränkungen

    Nur für Forschungszwecke einsetzbar
  • Validierung #029836 (Western Blotting)
    'Independent Validation' Siegel
    by
    Kinexus Bioinformatics Corporation
    No.
    #029836
    Datum
    22.06.2015
    Antigen
    Chargennummer
    251003/3
    Validierte Anwendung
    Western Blotting
    Positivkontrolle
    mouse brain, mouse kidney, mouse muscle, mouse spleen, mouse testes
    Negativkontrolle
    none [ubiquitously expressed]
    Bewertung
    A band was observed in the positive controls at the expected size (~42 kDa)
    'Independent Validation' Siegel
    Validierungsbilder
    Protokoll
    Primärantikörper
    • Antigen: Beta-Actin, (ACTB) (AA 2-16)
    • Catalog number: ABIN1742553
    • Lot number: 251003/3
    • Dilution: 1:1000
    Sekundärantikörper
    • Antibody: Donkey anti-Rabbit IgG Antibody (HRP)
    • Lot number: F0613
    • Dilution: 1:10,000
    Full Protocol
    • 1. Cell/tissue total protein lysates were boiled in 1X SDS Sample Buffer containing 1% SDS and 1.25% β-mercaptoethanol at 95°C for 5 minutes prior to loading.
    • 2. 15 μg of boiled lysate were loaded and resolved on a 12% SDS-polyacrylamide gel.
    • 3. The Precision Plus Protein™ All Blue Prestained Standards from BioRad (161-0373) were used as molecular mass markers.
    • 4. Proteins were transferred onto nitrocellulose membrane by tank transfer and protein transfer was confirmed with Ponceau S staining.
    • 5. The immunoblot membrane was blocked in 2.5% skim milk and 1.5% BSA solution in TTBS at room temperature for 60 minutes.
    • 6. The membrane was washed in TTBS twice for 5 minutes each.
    • 7. The membrane was immersed with the protein side up in the antibody solution in TBS and incubated overnight at 4°C with gentle agitation.
    • 8. The membrane was rinsed twice with TTBS.
    • 9. The membrane was washed in TTBS twice for 5 minutes each.
    • 10. The membrane was washed in TTBS once for 15 minutes.
    • 11. The membrane was incubated in the HRP-conjugated secondary antibody solution in TBS for 60 minutes at room temperature with gentle agitation.
    • 12. The membrane was rinsed twice with TTBS.
    • 13. The membrane was washed in TTBS twice for 5 minutes each.
    • 14. The membrane was washed in TTBS once for 15 minutes.
    • 15. Signals were detected by chemiluminescence (ECL). The blot was scanned for 160 seconds.
    • 16. The membrane was rinsed three times with TTBS.
    • 17. Incubated in Acidic Glycine Stripping Buffer at room temperature with gentle agitation for 3 times, 10 minutes each.
    • 18. The membrane was washed in TTBS 3 times for 5 minutes each.
    • 19. Repeated Steps 4-14 with the loading control antibody (for β3−Tubulin).
    • 20. Repeated Steps 4-14 with the loading control antibody and its matching secondary antibody. The blot was scanned for 160 seconds.
    Anmerkungen
    A protein band was observed at the expected target size of β−Actin of 42 kDa in all mouse extracts tested. Lower molecular weight bands of approximately 28-30 kDa were also observed in the mouse kidney and spleen tissue samples. Three main groups of actin have been identified. The β-actins and γ-actins co-exist in most cell types as components of the cytoskeleton, whereas the α-actin are found in muscle tissues. In our experience, we find that the level of actin does vary widely between different cell and tissue types.
  • Format

    Lyophilized

    Rekonstitution

    For reconstitution add 100 µL H2O to get a 1mg/ml solution of antibody in PBS. Then aliquot and store at -20 °C until use.

    Buffer

    PBS

    Handhabung

    Affinity purified antibodies are less robust than antisera, since protease inhibitors are also removed during purification. Hence, storage at 4 °C for prolonged periods (i.e. several weeks), is not recommended.

    Lagerung

    -20 °C

    Informationen zur Lagerung

    Unlabeled lyophilized antibodies are stable in this form without loss of quality at ambient temperatures for several weeks or even months. They can be stored at 4°C for several years. Lyophilized antibodies must not be stored in the freezer, they may be destroyed!
  • Lu, Ma, Zhuang, Zhang, Cheng, Shi: "Retinoic acid‑incorporated glycol chitosan nanoparticles inhibit the expression of Ezh2 in U118 and U138 human glioma cells." in: Molecular medicine reports, Vol. 12, Issue 5, pp. 6642-8, (2016) (PubMed).

    Baron, Blex, Rohrbeck, Rachakonda, Birnbaumer, Ahnert-Hilger, Brunk: "The α-subunit of the trimeric GTPase Go2 regulates axonal growth." in: Journal of neurochemistry, Vol. 124, Issue 6, pp. 782-94, (2013) (PubMed).

    Whitemarsh, Strathman, Chase, Stankewicz, Tepp, Johnson, Pellett: "Novel application of human neurons derived from induced pluripotent stem cells for highly sensitive botulinum neurotoxin detection." in: Toxicological sciences : an official journal of the Society of Toxicology, Vol. 126, Issue 2, pp. 426-35, (2012) (PubMed).

  • Target

    beta Actin (ACTB) (Actin, beta (ACTB))

    Andere Bezeichnung

    beta-Actin

    Pathways

    Myometrial Relaxation and Contraction, Cell-Cell Junction Organization, Maintenance of Protein Location, Phototransduction
Sie sind hier:
Chat with us!