TNC Antikörper

Produktnummer ABIN1742432

Der Ratte Monoklonal Anti-TNC-Antikörper wurde für WB, IHC und ICC validiert. Er ist geeignet, TNC in Proben von Ratte und Maus zu detektieren. Es sind 5+ Publikationen verfügbar.

Kurzübersicht für TNC Antikörper (ABIN1742432)

Target: TNC (Tenascin C (TNC))

Reaktivität: Ratte, Maus

Wirt: Ratte

Klonalität: Monoklonal

Konjugat: Dieser TNC Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC)

Klon: 578

Produktdetails anti-TNC Antikörper

Spezifität: Specific for tenascin-C splice variants carrying the FNIII D domain.

Aufreinigung: hybridoma supernatant

Immunogen: Glycopeptidase F-treated tenascin from mouse brain. Epitope FNIII D domain

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: 1:10.000 recommended dilution

Kommentare:

Tenascin-C variants detected by this antibody are downregulated during development and hardly detectable in adult animals

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Rekonstitution: For reconstitution add 200 µl H2O, then aliquot and store at -20°C until use.

Buffer: PBS

Handhabung: When hybridoma supernatant is reconstituted, we usually add small amounts of azide or thimerosal to prevent microbial growth. Ascites should be stored frozen (-20 °C). Monoclonals usually do not suffer from repetitive freeze-thawing but you may aliquot them into small samples to avoid too many freeze-thaw cycles.
Prolonged storage at 4 °C is not recommended! Unlike serum, ascites may contain proteases that will ultimately degrade the antibodies. Addition of protease inhibitors helps to slow degradation.

Lagerung: -20 °C

Informationen zur Lagerung: Unlabeled antibodies are stable in this form without loss of quality at ambient temperatures for several weeks or even months. They can be stored at 4 °C for several years.

Referenzen für anti-TNC Antikörper (ABIN1742432)

Siddiqui, Horvat-Bröcker, Faissner: "The glia-derived extracellular matrix glycoprotein tenascin-C promotes embryonic and postnatal retina axon outgrowth via the alternatively spliced fibronectin type III domain TNfnD." in: Neuron glia biology, Vol. 4, Issue 4, pp. 271-83, (2010) (PubMed).

Rigato, Garwood, Calco, Heck, Faivre-Sarrailh, Faissner: "Tenascin-C promotes neurite outgrowth of embryonic hippocampal neurons through the alternatively spliced fibronectin type III BD domains via activation of the cell adhesion molecule F3/contactin." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 22, Issue 15, pp. 6596-609, (2002) (PubMed).

Bartsch, Bartsch, Dörries, Faissner, Weller, Ekblom, Schachner: "Expression of tenascin in the developing and adult cerebellar cortex." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 12, Issue 3, pp. 736-49, (1992) (PubMed).

Stern, Norris, Bronner-Fraser, Carlson, Faissner, Keynes, Schachner: "J1/tenascin-related molecules are not responsible for the segmented pattern of neural crest cells or motor axons in the chick embryo." in: Development (Cambridge, England), Vol. 107, Issue 2, pp. 309-19, (1990) (PubMed).

Gatchalian, Schachner, Sanes: "Fibroblasts that proliferate near denervated synaptic sites in skeletal muscle synthesize the adhesive molecules tenascin(J1), N-CAM, fibronectin, and a heparan sulfate proteoglycan." in: The Journal of cell biology, Vol. 108, Issue 5, pp. 1873-90, (1989) (PubMed).

Details zu TNC

Target: TNC (Tenascin C (TNC))

Andere Bezeichnung: Tenascin-C

Hintergrund: Synonyms: TN-C, J1-200/220, Cytoactin

Pathways: Regulation of Muscle Cell Differentiation, Regulation of Cell Size, Skeletal Muscle Fiber Development