GAD65 Antikörper (N-Term)

Produktnummer ABIN1742419

Dieser Kaninchen Polyklonal Antikörper erkennt spezifisch GAD65 in WB, IHC, ELISA, IP, ICC und IHC (p). Er zeigt eine Reaktivität gegenüber Ratte und Maus und wurde in 2+ Publikationen erwähnt.

Kurzübersicht für GAD65 Antikörper (N-Term) (ABIN1742419)

Target: GAD65 (GAD2) (Glutamate Decarboxylase 2 (Pancreatic Islets and Brain, 65kDa) (GAD2))

Reaktivität: Ratte, Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser GAD65 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunoprecipitation (IP), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails anti-GAD65 Antikörper

Bindungsspezifität: N-Term

Verwendungszweck: Rabbit anti-GAD2 antibody

Spezifität: Specific for GAD 2 / GAD 65.

Kreuzreaktivität (Details): Reacts with: rat (P18088), mouse (P48318). Other species not tested yet.

Aufreinigung: affinity purified

Immunogen: Recombinant protein corresponding to the amino terminus of mouse GAD2 (UniProt Id: P48320)

Anwendungsinformationen

Applikationshinweise: WB:1 : 1000 , IP:yes ICC:1 : 2000, IHC:1 : 1000, IHC-P:1 : 1000, ELISA:yes

Kommentare:

ICC/IHC: The crude antiserum is recommended. ELISA: Suitable as detector antibody for sandwich-ELISA with ABIN1742417 as capture antibody (protocol for sandwich-ELISA).

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: For reconstitution add 50 μLH2O to get a 1 mg/mL solution in PBS.

Buffer: PBS. Albumin and azide were added for stabilization.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Do not freeze! Antibodies should be stored at +4°C when still lyophilized.

Lagerung: -20 °C,-80 °C,4 °C

Informationen zur Lagerung: Antibodies should be stored at +4°C when still lyophilized. Then aliquot and store at -20°C to -80°C until use. Albumin and azide were added for stabilization. Do not freeze!

Referenzen für anti-GAD65 Antikörper (ABIN1742419)

Du, Chazalon, Bestaven, Leste-Lasserre, Baufreton, Cazalets, Cho, Garret: "Early GABAergic transmission defects in the external globus pallidus and rest/activity rhythm alteration in a mouse model of Huntington's disease." in: Neuroscience, Vol. 329, pp. 363-79, (2016) (PubMed).

Saliba, Gu, Yan, Moss: "Blocking L-type voltage-gated Ca2+ channels with dihydropyridines reduces gamma-aminobutyric acid type A receptor expression and synaptic inhibition." in: The Journal of biological chemistry, Vol. 284, Issue 47, pp. 32544-50, (2009) (PubMed).

Details zu GAD65

Target: GAD65 (GAD2) (Glutamate Decarboxylase 2 (Pancreatic Islets and Brain, 65kDa) (GAD2))

Andere Bezeichnung: GAD2

Hintergrund:

Synonyms: Glutamic acid decarboxylase, GAD65, Glutamic acid decarboxylase 2, Glutamic acid decarboxylase 65

Antigen Information: GAD1 and 2 are involved in the synthesis of GABA, the major inhibitory neurotransmitter

UniProt: P48320