KCNK3 Antikörper (AA 251-411)

Produktnummer ABIN1741485

Dieses Anti-KCNK3-Antikörper ist ein Maus Monoklonal-Antikörper zur Detektion von KCNK3 in WB, IHC, ICC und IF. Geeignet für Ratte.

Kurzübersicht für KCNK3 Antikörper (AA 251-411) (ABIN1741485)

Target: KCNK3 (Potassium Channel, Subfamily K, Member 3 (KCNK3))

Reaktivität: Ratte

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser KCNK3 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF)

Klon: S374-48

Produktdetails anti-KCNK3 Antikörper

Bindungsspezifität: AA 251-411

Spezifität: Detects ~50 kDa. Does not cross-react with TASK3.

Kreuzreaktivität: Human, Maus, Ratte

Aufreinigung: Protein G Purified

Immunogen: Fusion protein amino acids 251-411 (cytoplasmic C-terminus) of rat Acid-sensitive potassium channel protein TASK or TASK1. Mouse: 96% identity (156/161 amino acids identical). Human: 76% identity (163/161 amino acids identical). <30% identity with TASK3.

Isotyp: IgG2b

Anwendungsinformationen

Applikationshinweise:

• WB (1:1000)
• ICC/IF (1:100)
• optimal dilutions for assays should be determined by the user.

Kommentare:

1 μg/ml of ABIN1741485 was sufficient for detection of TASK1 Potassium Channel in 20 μg of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: PBS pH 7.4, 50 % glycerol, 0.1 % sodium azide, Storage buffer may change when conjugated

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: -20°C

Details zu KCNK3

Target: KCNK3 (Potassium Channel, Subfamily K, Member 3 (KCNK3))

Andere Bezeichnung: KCNK3

Hintergrund: K+ channels are divided into three subclasses reflecting the number of transmembrane segments (TMS), which are designated 6TMS, 4TMS and 2TMS. Members of the 4TMS class contain two distinct pore regions and include TWIK, TREK, TRAAK and TASK. TASK channels are highly sensitive to external pH in the physiological range. TASK-1 is expressed in brain and in rat heart, with high levels of expression in the right atrium. TASK-2, mainly expressed in kidney, is localized in cortical distal tubules and collecting ducts, suggesting a role in renal K+ transport. TASK-3 from rat cerebellum shares 54 % identity with TASK-1, but less than 30 % identity with TASK-2 and other tandem pore K+ channels.

Gen-ID: 29553

NCBI Accession: NP_203694

UniProt: O54912