APAF1 Antikörper (C-Term)

Produktnummer ABIN1731833

Der Kaninchen Polyklonal anti-APAF1 Antikörper wird verwendet zum Nachweis von APAF1 in Proben von Human, Maus und Ratte. Er wurde validiert für WB, IHC, ELISA, ICC und IF.

Kurzübersicht für APAF1 Antikörper (C-Term) (ABIN1731833)

Target: APAF1 (Apoptotic Peptidase Activating Factor 1 (APAF1))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser APAF1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunocytochemistry (ICC), Immunofluorescence (IF)

Produktdetails anti-APAF1 Antikörper

Bindungsspezifität: AA 1158-1177, C-Term

Spezifität: Reacts with human, mouse, and rat Apaf1 (130 kDa).

Aufreinigung: Purified

Immunogen: Synthetic peptide, corresponding to amino acids 1158-1177 (C-terminus) of Human Apaf 1. The sequence of the immunogenic peptide differs from that of murine Apaf1 byone amino acid

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Working dilution: Optimal dilution should be determined by the end user.
The following are guidelines only:
IHC(10 - 20 μg/mL) WB(1 - 10 μg/mL) ELISA(1:100 - 1:2000)

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: PBS, pH 7.4

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Dilute in PBS or medium that is identical to that used in the assay system.

Lagerung: -20 °C

Informationen zur Lagerung: Stable for at least one year at -20°C. Avoid multiple freeze-thaw cycles.

Haltbarkeit: 12 months

Details zu APAF1

Target: APAF1 (Apoptotic Peptidase Activating Factor 1 (APAF1))

Andere Bezeichnung: Apaf1

Hintergrund: The mammalian homologues of the key cell death gene CED-4 in C. elegans has been identified recently from human and mouse and designated Apaf1 (for apoptosis proteaseactivating factor 1). Apaf1 binds to cytochrome c (Apaf2) and caspase-9 (Apaf3), which leads to caspase-9 activation. Activated caspase-9 in turn cleaves and activates caspase-3, one of the key proteases responsible for the proteolytic cleavage of many key proteins in apoptosis. Apaf1 can also associate with caspase-4 and caspase-8. Apaf1 is ubiquitously expressed in human tissues.

Apoptotic protease-activating factor 1, APAF-1 Gene name: APAF1, KIAA0413, CED4

Gen-ID: 317

UniProt: O14727

Pathways: p53 Signalweg, Apoptose, Caspase Kaskade in der Apoptose, Tube Formation, Positive Regulation of Endopeptidase Activity