GRP78 Antikörper

Produktnummer ABIN1721537

Der Maus Monoklonal Anti-GRP78-Antikörper wurde für WB und ELISA validiert. Er ist geeignet, GRP78 in Proben von Human, Ratte, Maus, Frosch, Xenopus laevis und Säugetier zu detektieren.

Kurzübersicht für GRP78 Antikörper (ABIN1721537)

Target: GRP78 (HSPA5) (Heat Shock 70kDa Protein 5 (Glucose-Regulated Protein, 78kDa) (HSPA5))

Reaktivität: Human, Ratte, Maus, Frosch, Xenopus laevis, Säugetier

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser GRP78 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA

Klon: 6H4-2G7

Produktdetails anti-GRP78 Antikörper

Spezifität: Reacts with human GRP78. Cross-reacts with mouse, rat, bovine, hamster, rabbit, monkey, xenopus and fungi (Cryphonectria parasitica) protein.

Aufreinigung: Purified (protein G)

Immunogen: His-tagged human GRP78 (accession no. NP_001156906.1)

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Working dilution: Optimal dilution should be determined by the end user.
The following are guidelines only :
WB(0.5 - 1.0 μg/mL)

Positive control: HeLa cell

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 2 mg/mL

Buffer: PBS, pH 7.4

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Dilute in PBS or medium which is identical to that used in the assay system.

Lagerung: -20 °C

Informationen zur Lagerung: For extended periods store in aliquots at -20°C. Antibodies are guaranteed for 6 months from date of receipt.

Haltbarkeit: 6 months

Details zu GRP78

Target: GRP78 (HSPA5) (Heat Shock 70kDa Protein 5 (Glucose-Regulated Protein, 78kDa) (HSPA5))

Andere Bezeichnung: GRP78

Hintergrund: Categorized as stress response proteins, BiPs play an important role in folding and assembly of nascent proteins and in scavenging of misfolded proteins in the endoplasmic reticulum lumen. Translation of BiP is directed by an internal ribosomal entry site (IRES) in the 5- nontranslated region of BiP mRNA. BiP IRES activity increases when cells are heat-stressed. GRP78 is also important in maintenance of cell homeostasis and the prevention of apoptosis. Levels of GRP78 protein are reduced in brains of Alzheimer's disease patients.

78 kDa glucose-regulated protein, GRP-78 Endoplasmic reticulum lumenal Ca(2+)-binding protein grp78 Heat shock 70 kDa protein 5 Immunoglobulin heavy chain-binding protein, BiP Gene name: HSPA5, GRP78

Gen-ID: 3309

UniProt: P11021

Pathways: Thyroid Hormone Synthesis, ER-Nucleus Signaling