AGO2 Antikörper

Produktnummer ABIN1720882

Dieser Kaninchen Polyklonal Antikörper detektiert spezifisch AGO2 in WB und IP. Es zeigt Reaktivität gegenüber Proben von Arabidopsis thaliana.

Kurzübersicht für AGO2 Antikörper (ABIN1720882)

Target: AGO2 (Argonaute 2 (AGO2))

Reaktivität: Arabidopsis thaliana

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser AGO2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunoprecipitation (IP)

Produktdetails anti-AGO2 Antikörper

Verwendungszweck: Anti-AGO2 | Argonaute 2

Spezifität: TAIR: At1g48410

Kreuzreaktivität (Details): Arabidopsis thaliana
Not reactive in: Chlamydomonas reinhardtii, Medicago truncatula, Nicotiana benthamiana, Populus sp., Solanum lycopersicum, Zea mays

Homologie: Capsella rubella,Solanum tuberosum

Aufreinigung: Immunogen affinity purified

Immunogen: KLH-conjugated synthetic peptide

Anwendungsinformationen

Applikationshinweise: 1 : 250-1 : 500 (WB)

Kommentare:

AGO expression may be tissue specific and using floral tissue is recommended where most of the AGOs are expressed the highest. Use of proteasome inhibitors as MG132 can help to stabilize AGO proteins during extraction procedure.The AGO1 antibody is extremely specific to AGO1 and does not cross-react with other antibodies. The evidence is 1) the peptide to which it was raised is at the very N-terminus of the protein and is not present in other AGOs 2) aAGO1 does not cross react with the AGOs which are overexpressed (AGO2, AGO3, AGO4, AGO5, AGO6, AGO9) using a western blot.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: For reconstitution add 50 μL of sterile water

Buffer: Immunogen affinity purified IgG in PBS pH 7.4.

Handhabung: Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube

Lagerung: -20 °C

Informationen zur Lagerung: Store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Details zu AGO2

Target: AGO2 (Argonaute 2 (AGO2))

Andere Bezeichnung: AGO2

Hintergrund: AGO2 belongs to a group of argonaute proteins which are catalytic component of the RNA-incudes silencing complex (RISC). This protein complex is responsible for the gene silencing (RNAi). AGO2 is probably involved in antiviral RNA silencing.

Molekulargewicht: 113 kDa

Gen-ID: 840016

UniProt: Q9SHF3

Pathways: Fc-epsilon Rezeptor Signalübertragung, Regulatorische RNA Pathways, EGFR Signaling Pathway, Neurotrophin Signalübertragung, Ribonucleoprotein Complex Subunit Organization