AGO2 Antikörper

Produktnummer ABIN1720882

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch AGO2 in WB. Er zeigt eine Reaktivität gegenüber Arabidopsis thaliana.

Kurzübersicht für AGO2 Antikörper (ABIN1720882)

Target: AGO2 (Argonaute 2 (AGO2))

Reaktivität: Arabidopsis thaliana

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser AGO2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB)

Produktdetails anti-AGO2 Antikörper

Spezifität: TAIR: At1g48410

Kreuzreaktivität (Details): Not reactive in: Chlamydomonas reinhardtii, Solanum lycopersicum, Zea mays

Homologie: Capsella rubella, Solanum tuberosum

Aufreinigung: serum

Immunogen: KLH-conjugated synthetic peptide, derived from Arabidopsis thaliana AGO2 protein, UniProt: Q9SHF3, TAIR: AT1G31280

Anwendungsinformationen

Applikationshinweise: 1: 250 - 1: 500 (WB)

Kommentare:

AGO expression may be tissue specific and using floral tissue is recommended where most of the AGOs are expressed the highest. Use of proteasome inhibitors as MG132 can help to stabilize AGO proteins during extraction procedure.The AGO1 antibody is extremely specific to AGO1 and does not cross-react with other antibodies. The evidence is 1) the peptide to which it was raised is at the very N-terminus of the protein and is not present in other AGOs 2) aAGO1 does not cross react with the AGOs which are overexpressed (AGO2, AGO3, AGO4, AGO5, AGO6, AGO9) using a western blot.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: For reconstitution add 50 μL of sterile water.

Handhabung: Avoid repeated freeze-thaw cycles.
Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Informationen zur Lagerung: Store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Details zu AGO2

Target: AGO2 (Argonaute 2 (AGO2))

Andere Bezeichnung: AGO2

Hintergrund: AGO1 belongs to a group of argonaute proteins which are catalytic component of the RNA-incudes silencing complex (RISC). This protein complex is responsible for the gene silencing (RNAi).

Molekulargewicht: expected: 116.4 kDa, appearent: 130 kDa

UniProt: Q9SHF3

Pathways: Fc-epsilon Rezeptor Signalübertragung, Regulatorische RNA Pathways, EGFR Signaling Pathway, Neurotrophin Signalübertragung, Ribonucleoprotein Complex Subunit Organization