AGO2 Antikörper (N-Term)

Produktnummer ABIN6259825

Produktdetails anti-AGO2 Antikörper

Target: AGO2 (Argonaute 2 (AGO2))

Bindungsspezifität: N-Term

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser AGO2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC)

Spezifität: AGO2 Antibody detects endogenous levels of total AGO2.

Homologie: Pig,Zebrafish,Bovine,Horse,Sheep,Rabbit,Xenopus

Aufreinigung: The antiserum was purified by peptide affinity chromatography using SulfoLink^TM Coupling Resin (Thermo Fisher Scientific).

Immunogen: A synthesized peptide derived from human AGO2, corresponding to a region within N-terminal amino acids.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB 1:500-1:2000, IHC 1:50-1:200

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Store at -20 °C. Stable for 12 months from date of receipt.

Haltbarkeit: 12 months

Details zu AGO2

Target: AGO2 (Argonaute 2 (AGO2))

Andere Bezeichnung: AGO2 (AGO2 Produkte)

Synonyme: EIF2C2 antikoerper, T19E23.7 antikoerper, T19E23_7 antikoerper, argonaute 2 antikoerper, Q10 antikoerper, Eif2c2 antikoerper, Argonaute2 antikoerper, eif2c1 antikoerper, eif2c2 antikoerper, 1110029L17Rik antikoerper, 2310051F07Rik antikoerper, AI225898 antikoerper, AL022874 antikoerper, AW546247 antikoerper, ENSMUSG00000072493 antikoerper, Gerp95 antikoerper, Gm10365 antikoerper, mKIAA4215 antikoerper, AGO2 antikoerper, AG02 antikoerper, AGO 2 antikoerper, Ago-2 antikoerper, Ago2 antikoerper, CG13452 antikoerper, CG7439 antikoerper, Dm Ago2 antikoerper, Dmel\\CG7439 antikoerper, ago antikoerper, ago-2 antikoerper, ago2 antikoerper, dAGO2 antikoerper, dAgo2 antikoerper, eIF2C2 antikoerper, argonaute 2, RISC catalytic component antikoerper, Argonaute family protein antikoerper, argonaute 2, RISC catalytic component L homeolog antikoerper, argonaute RISC catalytic subunit 2 antikoerper, argonaute RISC catalytic component 2 antikoerper, Argonaute 2 antikoerper, AGO2 antikoerper, Ago2 antikoerper, ago2.L antikoerper, ago2 antikoerper

Hintergrund:

Description: Required for RNA-mediated gene silencing (RNAi) by the RNA-induced silencing complex (RISC). The 'minimal RISC' appears to include AGO2 bound to a short guide RNA such as a microRNA (miRNA) or short interfering RNA (siRNA). These guide RNAs direct RISC to complementary mRNAs that are targets for RISC-mediated gene silencing. The precise mechanism of gene silencing depends on the degree of complementarity between the miRNA or siRNA and its target. Binding of RISC to a perfectly complementary mRNA generally results in silencing due to endonucleolytic cleavage of the mRNA specifically by AGO2. Binding of RISC to a partially complementary mRNA results in silencing through inhibition of translation, and this is independent of endonuclease activity. May inhibit translation initiation by binding to the 7-methylguanosine cap, thereby preventing the recruitment of the translation initiation factor eIF4-E. May also inhibit translation initiation via interaction with EIF6, which itself binds to the 60S ribosomal subunit and prevents its association with the 40S ribosomal subunit. The inhibition of translational initiation leads to the accumulation of the affected mRNA in cytoplasmic processing bodies (P-bodies), where mRNA degradation may subsequently occur. In some cases RISC-mediated translational repression is also observed for miRNAs that perfectly match the 3' untranslated region (3'-UTR). Can also up-regulate the translation of specific mRNAs under certain growth conditions. Binds to the AU element of the 3'-UTR of the TNF (TNF-alpha) mRNA and up-regulates translation under conditions of serum starvation. Also required for transcriptional gene silencing (TGS), in which short RNAs known as antigene RNAs or agRNAs direct the transcriptional repression of complementary promoter regions.

Gene: AGO2

Molekulargewicht: 90-95 kDa

Gen-ID: 27161

UniProt: Q9UKV8

Pathways: Fc-epsilon Rezeptor Signalübertragung, Regulatorische RNA Pathways, EGFR Signaling Pathway, Neurotrophin Signalübertragung, Ribonucleoprotein Complex Subunit Organization